Phage P1-Derived Artificial Chromosomes Facilitate Heterologous Expression of the FK506 Gene Cluster

We describe a procedure for the conjugative transfer of phage P1-derived Artificial Chromosome (PAC) library clones containing large natural product gene clusters (≥70 kilobases) to Streptomyces coelicolor strains that have been engineered for improved heterologous production of natural products. Th...

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Detalles Bibliográficos
Autores principales: Jones, Adam C., Gust, Bertolt, Kulik, Andreas, Heide, Lutz, Buttner, Mark J., Bibb, Mervyn J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708917/
https://www.ncbi.nlm.nih.gov/pubmed/23874942
http://dx.doi.org/10.1371/journal.pone.0069319

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708917/
https://www.ncbi.nlm.nih.gov/pubmed/23874942
http://dx.doi.org/10.1371/journal.pone.0069319

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