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Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System

The membrane protein FlhB is a highly conserved component of the flagellar secretion system, and it plays an active role in the regulation of protein export. In this study conserved properties of FlhB that are important for its function were investigated. Replacing the flhB gene (or part of the gene...

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Autores principales: Meshcheryakov, Vladimir A., Barker, Clive S., Kostyukova, Alla S., Samatey, Fadel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708937/
https://www.ncbi.nlm.nih.gov/pubmed/23874605
http://dx.doi.org/10.1371/journal.pone.0068384
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author Meshcheryakov, Vladimir A.
Barker, Clive S.
Kostyukova, Alla S.
Samatey, Fadel A.
author_facet Meshcheryakov, Vladimir A.
Barker, Clive S.
Kostyukova, Alla S.
Samatey, Fadel A.
author_sort Meshcheryakov, Vladimir A.
collection PubMed
description The membrane protein FlhB is a highly conserved component of the flagellar secretion system, and it plays an active role in the regulation of protein export. In this study conserved properties of FlhB that are important for its function were investigated. Replacing the flhB gene (or part of the gene) in Salmonella typhimurium with the flhB gene of the distantly related bacterium Aquifex aeolicus greatly reduces motility. However, motility can be restored to some extent by spontaneous mutations in the part of flhB gene coding for the cytoplasmic domain of Aquifex FlhB. Structural analysis suggests that these mutations destabilize the structure. The secondary structure and stability of the mutated cytoplasmic fragments of FlhB have been studied by circular dichroism spectroscopy. The results suggest that conformational flexibility could be important for FlhB function. An extragenic suppressor mutation in the fliS gene, which decreases the affinity of FliS to FliC, partially restores motility of the FlhB substitution mutants.
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spelling pubmed-37089372013-07-19 Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System Meshcheryakov, Vladimir A. Barker, Clive S. Kostyukova, Alla S. Samatey, Fadel A. PLoS One Research Article The membrane protein FlhB is a highly conserved component of the flagellar secretion system, and it plays an active role in the regulation of protein export. In this study conserved properties of FlhB that are important for its function were investigated. Replacing the flhB gene (or part of the gene) in Salmonella typhimurium with the flhB gene of the distantly related bacterium Aquifex aeolicus greatly reduces motility. However, motility can be restored to some extent by spontaneous mutations in the part of flhB gene coding for the cytoplasmic domain of Aquifex FlhB. Structural analysis suggests that these mutations destabilize the structure. The secondary structure and stability of the mutated cytoplasmic fragments of FlhB have been studied by circular dichroism spectroscopy. The results suggest that conformational flexibility could be important for FlhB function. An extragenic suppressor mutation in the fliS gene, which decreases the affinity of FliS to FliC, partially restores motility of the FlhB substitution mutants. Public Library of Science 2013-07-11 /pmc/articles/PMC3708937/ /pubmed/23874605 http://dx.doi.org/10.1371/journal.pone.0068384 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Meshcheryakov, Vladimir A.
Barker, Clive S.
Kostyukova, Alla S.
Samatey, Fadel A.
Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
title Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
title_full Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
title_fullStr Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
title_full_unstemmed Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
title_short Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
title_sort function of flhb, a membrane protein implicated in the bacterial flagellar type iii secretion system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708937/
https://www.ncbi.nlm.nih.gov/pubmed/23874605
http://dx.doi.org/10.1371/journal.pone.0068384
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