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Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory
We demonstrate the feasibility of using glass microbeads with a holographic barcode identifier to track DNA specimens in the molecular pathology laboratory. These beads can be added to peripheral blood specimens and are carried through automated DNA extraction protocols that use magnetic glass parti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709105/ https://www.ncbi.nlm.nih.gov/pubmed/23862106 http://dx.doi.org/10.7717/peerj.91 |
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author | Merker, Jason D. O’Grady, Naomi Gojenola, Linda Dao, Mai Lenta, Ross Yeakley, Joanne M. Schrijver, Iris |
author_facet | Merker, Jason D. O’Grady, Naomi Gojenola, Linda Dao, Mai Lenta, Ross Yeakley, Joanne M. Schrijver, Iris |
author_sort | Merker, Jason D. |
collection | PubMed |
description | We demonstrate the feasibility of using glass microbeads with a holographic barcode identifier to track DNA specimens in the molecular pathology laboratory. These beads can be added to peripheral blood specimens and are carried through automated DNA extraction protocols that use magnetic glass particles. We found that an adequate number of microbeads are consistently carried over during genomic DNA extraction to allow specimen identification, that the beads do not interfere with the performance of several different molecular assays, and that the beads and genomic DNA remain stable when stored together under regular storage conditions in the molecular pathology laboratory. The beads function as an internal, easily readable specimen barcode. This approach may be useful for identifying DNA specimens and reducing errors associated with molecular laboratory testing. |
format | Online Article Text |
id | pubmed-3709105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-37091052013-07-16 Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory Merker, Jason D. O’Grady, Naomi Gojenola, Linda Dao, Mai Lenta, Ross Yeakley, Joanne M. Schrijver, Iris PeerJ Pathology We demonstrate the feasibility of using glass microbeads with a holographic barcode identifier to track DNA specimens in the molecular pathology laboratory. These beads can be added to peripheral blood specimens and are carried through automated DNA extraction protocols that use magnetic glass particles. We found that an adequate number of microbeads are consistently carried over during genomic DNA extraction to allow specimen identification, that the beads do not interfere with the performance of several different molecular assays, and that the beads and genomic DNA remain stable when stored together under regular storage conditions in the molecular pathology laboratory. The beads function as an internal, easily readable specimen barcode. This approach may be useful for identifying DNA specimens and reducing errors associated with molecular laboratory testing. PeerJ Inc. 2013-07-02 /pmc/articles/PMC3709105/ /pubmed/23862106 http://dx.doi.org/10.7717/peerj.91 Text en © 2013 Merker et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Pathology Merker, Jason D. O’Grady, Naomi Gojenola, Linda Dao, Mai Lenta, Ross Yeakley, Joanne M. Schrijver, Iris Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory |
title | Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory |
title_full | Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory |
title_fullStr | Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory |
title_full_unstemmed | Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory |
title_short | Feasibility of using microbeads with holographic barcodes to track DNA specimens in the clinical molecular laboratory |
title_sort | feasibility of using microbeads with holographic barcodes to track dna specimens in the clinical molecular laboratory |
topic | Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709105/ https://www.ncbi.nlm.nih.gov/pubmed/23862106 http://dx.doi.org/10.7717/peerj.91 |
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