Looking at microbial metabolism by high-resolution (2)H-NMR spectroscopy

We analyzed the applicability of high-resolution (2)H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D(2)O contents. Such analysis i...

Descripción completa

Detalles Bibliográficos
Autores principales: Kutyshenko, Victor P., Beskaravayny, Petr M., Molchanov, Maxim V., Paskevich, Svetlana I., Prokhorov, Dmitry A., Uversky, Vladimir N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2013
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709107/
https://www.ncbi.nlm.nih.gov/pubmed/23862103
http://dx.doi.org/10.7717/peerj.101
Descripción
Sumario:We analyzed the applicability of high-resolution (2)H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D(2)O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.

Ejemplares similares