Cargando…
Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane
Reconstitution of membrane proteins in artificial membrane systems creates a platform for exploring their potential for pharmacological or biotechnological applications. Previously, we demonstrated amphiphilic block copolymers as promising building blocks for artificial membranes with long-term stab...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709162/ https://www.ncbi.nlm.nih.gov/pubmed/23846807 http://dx.doi.org/10.1038/srep02196 |
_version_ | 1782276718586757120 |
---|---|
author | Zhang, Xiaoyan Fu, Wangyang Palivan, Cornelia G. Meier, Wolfgang |
author_facet | Zhang, Xiaoyan Fu, Wangyang Palivan, Cornelia G. Meier, Wolfgang |
author_sort | Zhang, Xiaoyan |
collection | PubMed |
description | Reconstitution of membrane proteins in artificial membrane systems creates a platform for exploring their potential for pharmacological or biotechnological applications. Previously, we demonstrated amphiphilic block copolymers as promising building blocks for artificial membranes with long-term stability and tailorable structural parameters. However, the insertion of membrane proteins has not previously been realized in a large-area, stable, and solid-supported artificial membrane. Here, we show the first, preliminary model of a channel membrane protein that is functionally incorporated in a completely artificial polymer, tethered, solid-supported bilayer membrane (TSSBM). Unprecedented ionic transport characteristics that differ from previous results on protein insertion into planar, free-standing membranes, are identified. Our findings mark a change in understanding protein insertion and ion flow within natural channel proteins when inserted in an artificial TSSBM, thus holding great potential for numerous applications such as drug screening, trace analyzing, and biosensing. |
format | Online Article Text |
id | pubmed-3709162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-37091622013-07-12 Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane Zhang, Xiaoyan Fu, Wangyang Palivan, Cornelia G. Meier, Wolfgang Sci Rep Article Reconstitution of membrane proteins in artificial membrane systems creates a platform for exploring their potential for pharmacological or biotechnological applications. Previously, we demonstrated amphiphilic block copolymers as promising building blocks for artificial membranes with long-term stability and tailorable structural parameters. However, the insertion of membrane proteins has not previously been realized in a large-area, stable, and solid-supported artificial membrane. Here, we show the first, preliminary model of a channel membrane protein that is functionally incorporated in a completely artificial polymer, tethered, solid-supported bilayer membrane (TSSBM). Unprecedented ionic transport characteristics that differ from previous results on protein insertion into planar, free-standing membranes, are identified. Our findings mark a change in understanding protein insertion and ion flow within natural channel proteins when inserted in an artificial TSSBM, thus holding great potential for numerous applications such as drug screening, trace analyzing, and biosensing. Nature Publishing Group 2013-07-12 /pmc/articles/PMC3709162/ /pubmed/23846807 http://dx.doi.org/10.1038/srep02196 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Zhang, Xiaoyan Fu, Wangyang Palivan, Cornelia G. Meier, Wolfgang Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane |
title | Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane |
title_full | Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane |
title_fullStr | Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane |
title_full_unstemmed | Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane |
title_short | Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane |
title_sort | natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709162/ https://www.ncbi.nlm.nih.gov/pubmed/23846807 http://dx.doi.org/10.1038/srep02196 |
work_keys_str_mv | AT zhangxiaoyan naturalchannelproteininsertsandfunctionsinacompletelyartificialsolidsupportedbilayermembrane AT fuwangyang naturalchannelproteininsertsandfunctionsinacompletelyartificialsolidsupportedbilayermembrane AT palivancorneliag naturalchannelproteininsertsandfunctionsinacompletelyartificialsolidsupportedbilayermembrane AT meierwolfgang naturalchannelproteininsertsandfunctionsinacompletelyartificialsolidsupportedbilayermembrane |