Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimiz...

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Detalles Bibliográficos
Autores principales: Merlos Rodrigo, Miguel Angel, Cernei, Natalia, Kominkova, Marketa, Zitka, Ondrej, Beklova, Miroslava, Zehnalek, Josef, Kizek, Rene, Adam, Vojtech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709318/
https://www.ncbi.nlm.nih.gov/pubmed/23538727
http://dx.doi.org/10.3390/ijerph10041304
Descripción
Sumario:In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL(−1) PC2 and 100 µg·mL(−1) of CdCl(2). The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.

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