Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimiz...

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Autores principales: Merlos Rodrigo, Miguel Angel, Cernei, Natalia, Kominkova, Marketa, Zitka, Ondrej, Beklova, Miroslava, Zehnalek, Josef, Kizek, Rene, Adam, Vojtech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709318/
https://www.ncbi.nlm.nih.gov/pubmed/23538727
http://dx.doi.org/10.3390/ijerph10041304
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author Merlos Rodrigo, Miguel Angel
Cernei, Natalia
Kominkova, Marketa
Zitka, Ondrej
Beklova, Miroslava
Zehnalek, Josef
Kizek, Rene
Adam, Vojtech
author_facet Merlos Rodrigo, Miguel Angel
Cernei, Natalia
Kominkova, Marketa
Zitka, Ondrej
Beklova, Miroslava
Zehnalek, Josef
Kizek, Rene
Adam, Vojtech
author_sort Merlos Rodrigo, Miguel Angel
collection PubMed
description In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL(−1) PC2 and 100 µg·mL(−1) of CdCl(2). The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.
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spelling pubmed-37093182013-07-12 Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes Merlos Rodrigo, Miguel Angel Cernei, Natalia Kominkova, Marketa Zitka, Ondrej Beklova, Miroslava Zehnalek, Josef Kizek, Rene Adam, Vojtech Int J Environ Res Public Health Article In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL(−1) PC2 and 100 µg·mL(−1) of CdCl(2). The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes. MDPI 2013-03-28 2013-04 /pmc/articles/PMC3709318/ /pubmed/23538727 http://dx.doi.org/10.3390/ijerph10041304 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Merlos Rodrigo, Miguel Angel
Cernei, Natalia
Kominkova, Marketa
Zitka, Ondrej
Beklova, Miroslava
Zehnalek, Josef
Kizek, Rene
Adam, Vojtech
Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes
title Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes
title_full Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes
title_fullStr Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes
title_full_unstemmed Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes
title_short Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes
title_sort ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (ii) complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709318/
https://www.ncbi.nlm.nih.gov/pubmed/23538727
http://dx.doi.org/10.3390/ijerph10041304
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