Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000

Bacteria contain small non-coding RNAs (ncRNAs) that are responsible for altering transcription, translation or mRNA stability. ncRNAs are important because they regulate virulence factors and susceptibility to various stresses. Here, the regulation of a recently described ncRNA of Pseudomonas syrin...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, So Hae, Butcher, Bronwyn G., Anderson, Zoe, Pellegrini, Nola, Bao, Zhongmeng, D’Amico, Katherine, Filiatrault, Melanie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2013
Materias:
Cell and Molecular Biology of Microbes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709562/
https://www.ncbi.nlm.nih.gov/pubmed/23258266
http://dx.doi.org/10.1099/mic.0.063826-0
_version_ 1782276769142800384
author Park, So Hae
Butcher, Bronwyn G.
Anderson, Zoe
Pellegrini, Nola
Bao, Zhongmeng
D’Amico, Katherine
Filiatrault, Melanie J.
author_facet Park, So Hae
Butcher, Bronwyn G.
Anderson, Zoe
Pellegrini, Nola
Bao, Zhongmeng
D’Amico, Katherine
Filiatrault, Melanie J.
author_sort Park, So Hae
collection PubMed
description Bacteria contain small non-coding RNAs (ncRNAs) that are responsible for altering transcription, translation or mRNA stability. ncRNAs are important because they regulate virulence factors and susceptibility to various stresses. Here, the regulation of a recently described ncRNA of Pseudomonas syringae pv. tomato DC3000, P16, was investigated. We determined that RpoS regulates the expression of P16. We found that deletion of P16 results in increased sensitivity to hydrogen peroxide compared to the wild-type strain, suggesting that P16 plays a role in the bacteria’s susceptibility to oxidative stress. Additionally the P16 mutant displayed enhanced resistance to heat stress. Our findings provide new information on the regulation and role of this ncRNA in P. syringae.
format Online
Article
Text
id pubmed-3709562
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Society for General Microbiology
record_format MEDLINE/PubMed
spelling pubmed-37095622013-07-24 Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000 Park, So Hae Butcher, Bronwyn G. Anderson, Zoe Pellegrini, Nola Bao, Zhongmeng D’Amico, Katherine Filiatrault, Melanie J. Microbiology (Reading) Cell and Molecular Biology of Microbes Bacteria contain small non-coding RNAs (ncRNAs) that are responsible for altering transcription, translation or mRNA stability. ncRNAs are important because they regulate virulence factors and susceptibility to various stresses. Here, the regulation of a recently described ncRNA of Pseudomonas syringae pv. tomato DC3000, P16, was investigated. We determined that RpoS regulates the expression of P16. We found that deletion of P16 results in increased sensitivity to hydrogen peroxide compared to the wild-type strain, suggesting that P16 plays a role in the bacteria’s susceptibility to oxidative stress. Additionally the P16 mutant displayed enhanced resistance to heat stress. Our findings provide new information on the regulation and role of this ncRNA in P. syringae. Society for General Microbiology 2013-02 /pmc/articles/PMC3709562/ /pubmed/23258266 http://dx.doi.org/10.1099/mic.0.063826-0 Text en © 2013 SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cell and Molecular Biology of Microbes
Park, So Hae
Butcher, Bronwyn G.
Anderson, Zoe
Pellegrini, Nola
Bao, Zhongmeng
D’Amico, Katherine
Filiatrault, Melanie J.
Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000
title Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000
title_full Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000
title_fullStr Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000
title_full_unstemmed Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000
title_short Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000
title_sort analysis of the small rna p16/rgsa in the plant pathogen pseudomonas syringae pv. tomato strain dc3000
topic Cell and Molecular Biology of Microbes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709562/
https://www.ncbi.nlm.nih.gov/pubmed/23258266
http://dx.doi.org/10.1099/mic.0.063826-0
work_keys_str_mv AT parksohae analysisofthesmallrnap16rgsaintheplantpathogenpseudomonassyringaepvtomatostraindc3000
AT butcherbronwyng analysisofthesmallrnap16rgsaintheplantpathogenpseudomonassyringaepvtomatostraindc3000
AT andersonzoe analysisofthesmallrnap16rgsaintheplantpathogenpseudomonassyringaepvtomatostraindc3000
AT pellegrininola analysisofthesmallrnap16rgsaintheplantpathogenpseudomonassyringaepvtomatostraindc3000
AT baozhongmeng analysisofthesmallrnap16rgsaintheplantpathogenpseudomonassyringaepvtomatostraindc3000
AT damicokatherine analysisofthesmallrnap16rgsaintheplantpathogenpseudomonassyringaepvtomatostraindc3000
AT filiatraultmelaniej analysisofthesmallrnap16rgsaintheplantpathogenpseudomonassyringaepvtomatostraindc3000

Ejemplares similares