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Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms

Rotaviruses (RVs) cause acute gastroenteritis in infants and young children, and are globally distributed. Within the infected host cell, RVs establish replication complexes in viroplasms (‘viral factories’) to which lipid droplet organelles are recruited. To further understand this recently discove...

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Autores principales: Gaunt, Eleanor R., Zhang, Qifeng, Cheung, Winsome, Wakelam, Michael J. O., Lever, Andrew M. L., Desselberger, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709634/
https://www.ncbi.nlm.nih.gov/pubmed/23515026
http://dx.doi.org/10.1099/vir.0.049635-0
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author Gaunt, Eleanor R.
Zhang, Qifeng
Cheung, Winsome
Wakelam, Michael J. O.
Lever, Andrew M. L.
Desselberger, Ulrich
author_facet Gaunt, Eleanor R.
Zhang, Qifeng
Cheung, Winsome
Wakelam, Michael J. O.
Lever, Andrew M. L.
Desselberger, Ulrich
author_sort Gaunt, Eleanor R.
collection PubMed
description Rotaviruses (RVs) cause acute gastroenteritis in infants and young children, and are globally distributed. Within the infected host cell, RVs establish replication complexes in viroplasms (‘viral factories’) to which lipid droplet organelles are recruited. To further understand this recently discovered phenomenon, the lipidomes of RV-infected and uninfected MA104 cells were investigated. Cell lysates were subjected to equilibrium ultracentrifugation through iodixanol gradients. Fourteen different classes of lipids were differentiated by mass spectrometry. The concentrations of virtually all lipids were elevated in RV-infected cells. Fractions of low density (1.11–1.15 g ml(−1)), in which peaks of the RV dsRNA genome and lipid droplet- and viroplasm-associated proteins were observed, contained increased amounts of lipids typically found concentrated in the cellular organelle lipid droplets, confirming the close interaction of lipid droplets with viroplasms. A decrease in the ratio of the amounts of surface to internal components of lipid droplets upon RV infection suggested that the lipid droplet–viroplasm complexes became enlarged.
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spelling pubmed-37096342013-07-24 Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms Gaunt, Eleanor R. Zhang, Qifeng Cheung, Winsome Wakelam, Michael J. O. Lever, Andrew M. L. Desselberger, Ulrich J Gen Virol Animal Rotaviruses (RVs) cause acute gastroenteritis in infants and young children, and are globally distributed. Within the infected host cell, RVs establish replication complexes in viroplasms (‘viral factories’) to which lipid droplet organelles are recruited. To further understand this recently discovered phenomenon, the lipidomes of RV-infected and uninfected MA104 cells were investigated. Cell lysates were subjected to equilibrium ultracentrifugation through iodixanol gradients. Fourteen different classes of lipids were differentiated by mass spectrometry. The concentrations of virtually all lipids were elevated in RV-infected cells. Fractions of low density (1.11–1.15 g ml(−1)), in which peaks of the RV dsRNA genome and lipid droplet- and viroplasm-associated proteins were observed, contained increased amounts of lipids typically found concentrated in the cellular organelle lipid droplets, confirming the close interaction of lipid droplets with viroplasms. A decrease in the ratio of the amounts of surface to internal components of lipid droplets upon RV infection suggested that the lipid droplet–viroplasm complexes became enlarged. Society for General Microbiology 2013-07 /pmc/articles/PMC3709634/ /pubmed/23515026 http://dx.doi.org/10.1099/vir.0.049635-0 Text en © 2013 SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Animal
Gaunt, Eleanor R.
Zhang, Qifeng
Cheung, Winsome
Wakelam, Michael J. O.
Lever, Andrew M. L.
Desselberger, Ulrich
Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms
title Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms
title_full Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms
title_fullStr Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms
title_full_unstemmed Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms
title_short Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms
title_sort lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709634/
https://www.ncbi.nlm.nih.gov/pubmed/23515026
http://dx.doi.org/10.1099/vir.0.049635-0
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