Cargando…

Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice

Previous in vitro and in vivo studies have shown that sodium orthovanadate (vanadate), an inorganic vanadium compound, could effectively suppress radiation-induced p53-mediated apoptosis via both transcription-dependent and transcription-independent pathways. As a potent radiation protector administ...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Bing, Tanaka, Kaoru, Morita, Akinori, Ninomiya, Yasuharu, Maruyama, Kouichi, Fujita, Kazuko, Hosoi, Yoshio, Nenoi, Mitsuru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709668/
https://www.ncbi.nlm.nih.gov/pubmed/23349341
http://dx.doi.org/10.1093/jrr/rrs140
_version_ 1782276778529652736
author Wang, Bing
Tanaka, Kaoru
Morita, Akinori
Ninomiya, Yasuharu
Maruyama, Kouichi
Fujita, Kazuko
Hosoi, Yoshio
Nenoi, Mitsuru
author_facet Wang, Bing
Tanaka, Kaoru
Morita, Akinori
Ninomiya, Yasuharu
Maruyama, Kouichi
Fujita, Kazuko
Hosoi, Yoshio
Nenoi, Mitsuru
author_sort Wang, Bing
collection PubMed
description Previous in vitro and in vivo studies have shown that sodium orthovanadate (vanadate), an inorganic vanadium compound, could effectively suppress radiation-induced p53-mediated apoptosis via both transcription-dependent and transcription-independent pathways. As a potent radiation protector administered at a dose of 20 mg/kg body weight (20 mg/kg) prior to total body irradiation (TBI) by intra-peritoneal (ip) injection, it completely protected mice from hematopoietic syndrome and partially from gastrointestinal syndrome. In the present study, radiation mitigation effects from vanadate were investigated by ip injection of vanadate after TBI in mice. Results showed that a single administration of vanadate at a dose of 20 mg/kg markedly improved the 30-day survival rate and the peripheral blood hemogram, relieved bone marrow aplasia and decreased occurrence of the bone marrow micronucleated erythrocytes in the surviving animals. The dose reduction factor was 1.2 when a single dose of 20 mg/kg was administered 15 min after TBI in mice using the 30-day survival test as the endpoint. Results also showed that either doubling the vanadate dose (40 mg/kg) in a single administration or continuing the vanadate treatment (after a single administration at 20 mg/kg) from the following day at a dose of 5 mg/kg per day for 4 consecutive days further significantly improved the efficacy for rescuing bone marrow failure in the 30-day survival test. Taken together, these findings indicate that vanadate would be a potent mitigator suppressing the acute lethality (hematopoietic syndrome) and minimizing the detrimental effects (anhematopoiesis and delayed genotoxic effects) induced by TBI in mice.
format Online
Article
Text
id pubmed-3709668
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-37096682013-07-15 Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice Wang, Bing Tanaka, Kaoru Morita, Akinori Ninomiya, Yasuharu Maruyama, Kouichi Fujita, Kazuko Hosoi, Yoshio Nenoi, Mitsuru J Radiat Res Biology Previous in vitro and in vivo studies have shown that sodium orthovanadate (vanadate), an inorganic vanadium compound, could effectively suppress radiation-induced p53-mediated apoptosis via both transcription-dependent and transcription-independent pathways. As a potent radiation protector administered at a dose of 20 mg/kg body weight (20 mg/kg) prior to total body irradiation (TBI) by intra-peritoneal (ip) injection, it completely protected mice from hematopoietic syndrome and partially from gastrointestinal syndrome. In the present study, radiation mitigation effects from vanadate were investigated by ip injection of vanadate after TBI in mice. Results showed that a single administration of vanadate at a dose of 20 mg/kg markedly improved the 30-day survival rate and the peripheral blood hemogram, relieved bone marrow aplasia and decreased occurrence of the bone marrow micronucleated erythrocytes in the surviving animals. The dose reduction factor was 1.2 when a single dose of 20 mg/kg was administered 15 min after TBI in mice using the 30-day survival test as the endpoint. Results also showed that either doubling the vanadate dose (40 mg/kg) in a single administration or continuing the vanadate treatment (after a single administration at 20 mg/kg) from the following day at a dose of 5 mg/kg per day for 4 consecutive days further significantly improved the efficacy for rescuing bone marrow failure in the 30-day survival test. Taken together, these findings indicate that vanadate would be a potent mitigator suppressing the acute lethality (hematopoietic syndrome) and minimizing the detrimental effects (anhematopoiesis and delayed genotoxic effects) induced by TBI in mice. Oxford University Press 2013-07 2013-01-24 /pmc/articles/PMC3709668/ /pubmed/23349341 http://dx.doi.org/10.1093/jrr/rrs140 Text en © The Author 2013. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biology
Wang, Bing
Tanaka, Kaoru
Morita, Akinori
Ninomiya, Yasuharu
Maruyama, Kouichi
Fujita, Kazuko
Hosoi, Yoshio
Nenoi, Mitsuru
Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice
title Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice
title_full Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice
title_fullStr Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice
title_full_unstemmed Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice
title_short Sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice
title_sort sodium orthovanadate (vanadate), a potent mitigator of radiation-induced damage to the hematopoietic system in mice
topic Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709668/
https://www.ncbi.nlm.nih.gov/pubmed/23349341
http://dx.doi.org/10.1093/jrr/rrs140
work_keys_str_mv AT wangbing sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice
AT tanakakaoru sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice
AT moritaakinori sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice
AT ninomiyayasuharu sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice
AT maruyamakouichi sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice
AT fujitakazuko sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice
AT hosoiyoshio sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice
AT nenoimitsuru sodiumorthovanadatevanadateapotentmitigatorofradiationinduceddamagetothehematopoieticsysteminmice