Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2

The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologousl...

Descripción completa

Detalles Bibliográficos
Autores principales: Shang, Shufeng, Jiang, Jun, Deng, Yiqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709703/
https://www.ncbi.nlm.nih.gov/pubmed/23702848
http://dx.doi.org/10.3390/ijms140610809
_version_ 1782276784022093824
author Shang, Shufeng
Jiang, Jun
Deng, Yiqun
author_facet Shang, Shufeng
Jiang, Jun
Deng, Yiqun
author_sort Shang, Shufeng
collection PubMed
description The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3′OH-T-2 by the 3′-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5α further confirm that CYP1A5 can convert T-2 into TC-1 (3′OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.
format Online
Article
Text
id pubmed-3709703
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-37097032013-07-12 Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2 Shang, Shufeng Jiang, Jun Deng, Yiqun Int J Mol Sci Article The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3′OH-T-2 by the 3′-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5α further confirm that CYP1A5 can convert T-2 into TC-1 (3′OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens. Molecular Diversity Preservation International (MDPI) 2013-05-23 /pmc/articles/PMC3709703/ /pubmed/23702848 http://dx.doi.org/10.3390/ijms140610809 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Shang, Shufeng
Jiang, Jun
Deng, Yiqun
Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2
title Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2
title_full Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2
title_fullStr Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2
title_full_unstemmed Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2
title_short Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3′OH-T-2
title_sort chicken cytochrome p450 1a5 is the key enzyme for metabolizing t-2 toxin to 3′oh-t-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709703/
https://www.ncbi.nlm.nih.gov/pubmed/23702848
http://dx.doi.org/10.3390/ijms140610809
work_keys_str_mv AT shangshufeng chickencytochromep4501a5isthekeyenzymeformetabolizingt2toxinto3oht2
AT jiangjun chickencytochromep4501a5isthekeyenzymeformetabolizingt2toxinto3oht2
AT dengyiqun chickencytochromep4501a5isthekeyenzymeformetabolizingt2toxinto3oht2

Ejemplares similares