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Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization

Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. Although several of the mechanisms involved in disease progression have been discovered, the molecular response of...

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Autores principales: Xu, Liang, Liu, Zhen-Yu, Zhang, Kai, Lu, Quan, Liang, Jun, Zhang, Xing-Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709736/
https://www.ncbi.nlm.nih.gov/pubmed/23759987
http://dx.doi.org/10.3390/ijms140611356
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author Xu, Liang
Liu, Zhen-Yu
Zhang, Kai
Lu, Quan
Liang, Jun
Zhang, Xing-Yao
author_facet Xu, Liang
Liu, Zhen-Yu
Zhang, Kai
Lu, Quan
Liang, Jun
Zhang, Xing-Yao
author_sort Xu, Liang
collection PubMed
description Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. Although several of the mechanisms involved in disease progression have been discovered, the molecular response of Pinus massoniana to PWN infection has not been explored. We constructed four subtractive suppression hybridization cDNA libraries by taking time-course samples from PWN-inoculated Masson pine trees. One-hundred forty-four significantly differentially expressed sequence tags (ESTs) were identified, and 124 high-quality sequences with transcriptional features were selected for gene ontology (GO) and individual gene analyses. There were marked differences in the types of transcripts, as well as in the timing and levels of transcript expression in the pine trees following PWN inoculation. Genes involved in signal transduction, transcription and translation and secondary metabolism were highly expressed after 24 h and 72 h, while stress response genes were highly expressed only after 72 h. Certain transcripts responding to PWN infection were discriminative; pathogenesis and cell wall-related genes were more abundant, while detoxification or redox process-related genes were less abundant. This study provides new insights into the molecular mechanisms that control the biochemical and physiological responses of pine trees to PWN infection, particularly during the initial stage of infection.
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spelling pubmed-37097362013-07-12 Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization Xu, Liang Liu, Zhen-Yu Zhang, Kai Lu, Quan Liang, Jun Zhang, Xing-Yao Int J Mol Sci Article Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. Although several of the mechanisms involved in disease progression have been discovered, the molecular response of Pinus massoniana to PWN infection has not been explored. We constructed four subtractive suppression hybridization cDNA libraries by taking time-course samples from PWN-inoculated Masson pine trees. One-hundred forty-four significantly differentially expressed sequence tags (ESTs) were identified, and 124 high-quality sequences with transcriptional features were selected for gene ontology (GO) and individual gene analyses. There were marked differences in the types of transcripts, as well as in the timing and levels of transcript expression in the pine trees following PWN inoculation. Genes involved in signal transduction, transcription and translation and secondary metabolism were highly expressed after 24 h and 72 h, while stress response genes were highly expressed only after 72 h. Certain transcripts responding to PWN infection were discriminative; pathogenesis and cell wall-related genes were more abundant, while detoxification or redox process-related genes were less abundant. This study provides new insights into the molecular mechanisms that control the biochemical and physiological responses of pine trees to PWN infection, particularly during the initial stage of infection. Molecular Diversity Preservation International (MDPI) 2013-05-28 /pmc/articles/PMC3709736/ /pubmed/23759987 http://dx.doi.org/10.3390/ijms140611356 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Xu, Liang
Liu, Zhen-Yu
Zhang, Kai
Lu, Quan
Liang, Jun
Zhang, Xing-Yao
Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization
title Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization
title_full Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization
title_fullStr Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization
title_full_unstemmed Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization
title_short Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization
title_sort characterization of the pinus massoniana transcriptional response to bursaphelenchus xylophilus infection using suppression subtractive hybridization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709736/
https://www.ncbi.nlm.nih.gov/pubmed/23759987
http://dx.doi.org/10.3390/ijms140611356
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