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Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane

The aim of this study was to compare the anti-adhesion efficacy of a bi-layer electrospun fibrous membrane consisting of hyaluronic acid-loaded poly(ɛ-caprolactone) (PCL) fibrous membrane as the inner layer and PCL fibrous membrane as the outer layer with a single-layer PCL electrospun fibrous membr...

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Detalles Bibliográficos
Autores principales: Jiang, Shichao, Wang, Wei, Yan, Hede, Fan, Cunyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709760/
https://www.ncbi.nlm.nih.gov/pubmed/23736693
http://dx.doi.org/10.3390/ijms140611861
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author Jiang, Shichao
Wang, Wei
Yan, Hede
Fan, Cunyi
author_facet Jiang, Shichao
Wang, Wei
Yan, Hede
Fan, Cunyi
author_sort Jiang, Shichao
collection PubMed
description The aim of this study was to compare the anti-adhesion efficacy of a bi-layer electrospun fibrous membrane consisting of hyaluronic acid-loaded poly(ɛ-caprolactone) (PCL) fibrous membrane as the inner layer and PCL fibrous membrane as the outer layer with a single-layer PCL electrospun fibrous membrane in a rat cecum abrasion model. The rat model utilized a cecal abrasion and abdominal wall insult surgical protocol. The bi-layer and PCL membranes were applied between the cecum and the abdominal wall, respectively. Control animals did not receive any treatment. After postoperative day 14, a visual semiquantitative grading scale was used to grade the extent of adhesion. Histological analysis was performed to reveal the features of adhesion tissues. Bi-layer membrane treated animals showed significantly lower adhesion scores than control animals (p < 0.05) and a lower adhesion score compared with the PCL membrane. Histological analysis of the bi-layer membrane treated rat rarely demonstrated tissue adhesion while that of the PCL membrane treated rat and control rat showed loose and dense adhesion tissues, respectively. Bi-layer membrane can efficiently prevent adhesion formation in abdominal cavity and showed a significantly decreased adhesion tissue formation compared with the control.
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spelling pubmed-37097602013-07-12 Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane Jiang, Shichao Wang, Wei Yan, Hede Fan, Cunyi Int J Mol Sci Article The aim of this study was to compare the anti-adhesion efficacy of a bi-layer electrospun fibrous membrane consisting of hyaluronic acid-loaded poly(ɛ-caprolactone) (PCL) fibrous membrane as the inner layer and PCL fibrous membrane as the outer layer with a single-layer PCL electrospun fibrous membrane in a rat cecum abrasion model. The rat model utilized a cecal abrasion and abdominal wall insult surgical protocol. The bi-layer and PCL membranes were applied between the cecum and the abdominal wall, respectively. Control animals did not receive any treatment. After postoperative day 14, a visual semiquantitative grading scale was used to grade the extent of adhesion. Histological analysis was performed to reveal the features of adhesion tissues. Bi-layer membrane treated animals showed significantly lower adhesion scores than control animals (p < 0.05) and a lower adhesion score compared with the PCL membrane. Histological analysis of the bi-layer membrane treated rat rarely demonstrated tissue adhesion while that of the PCL membrane treated rat and control rat showed loose and dense adhesion tissues, respectively. Bi-layer membrane can efficiently prevent adhesion formation in abdominal cavity and showed a significantly decreased adhesion tissue formation compared with the control. Molecular Diversity Preservation International (MDPI) 2013-06-04 /pmc/articles/PMC3709760/ /pubmed/23736693 http://dx.doi.org/10.3390/ijms140611861 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Jiang, Shichao
Wang, Wei
Yan, Hede
Fan, Cunyi
Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane
title Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane
title_full Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane
title_fullStr Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane
title_full_unstemmed Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane
title_short Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane
title_sort prevention of intra-abdominal adhesion by bi-layer electrospun membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709760/
https://www.ncbi.nlm.nih.gov/pubmed/23736693
http://dx.doi.org/10.3390/ijms140611861
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