Cargando…

Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)

Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior variet...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Hyun Ji, Jung, Won Yong, Lee, Sang Sook, Song, Jun Ho, Kwon, Suk-Yoon, Kim, HyeRan, Kim, ChulWook, Ahn, Jun Cheul, Cho, Hye Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709761/
https://www.ncbi.nlm.nih.gov/pubmed/23736694
http://dx.doi.org/10.3390/ijms140611871
_version_ 1782276797277143040
author Park, Hyun Ji
Jung, Won Yong
Lee, Sang Sook
Song, Jun Ho
Kwon, Suk-Yoon
Kim, HyeRan
Kim, ChulWook
Ahn, Jun Cheul
Cho, Hye Sun
author_facet Park, Hyun Ji
Jung, Won Yong
Lee, Sang Sook
Song, Jun Ho
Kwon, Suk-Yoon
Kim, HyeRan
Kim, ChulWook
Ahn, Jun Cheul
Cho, Hye Sun
author_sort Park, Hyun Ji
collection PubMed
description Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. We compared heat-responsive gene expression between a heat-tolerant cabbage line (HTCL), “HO”, and a heat-sensitive cabbage line (HSCL), “JK”, by Genechip assay. Expression levels of specific heat stress-related genes were increased in response to high-temperature stress, according to Genechip assays. We performed quantitative RT-PCR (qRT-PCR) to compare expression levels of these heat stress-related genes in four HTCLs and four HSCLs. Transcript levels for heat shock protein BoHsp70 and transcription factor BoGRAS (SCL13) were more strongly expressed only in all HTCLs compared to all HSCLs, showing much lower level expressions at the young plant stage under heat stress (HS). Thus, we suggest that expression levels of these genes may be early selection markers for HTCLs in cabbage breeding. In addition, several genes that are involved in the secondary metabolite pathway were differentially regulated in HTCL and HSCL exposed to heat stress.
format Online
Article
Text
id pubmed-3709761
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-37097612013-07-12 Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.) Park, Hyun Ji Jung, Won Yong Lee, Sang Sook Song, Jun Ho Kwon, Suk-Yoon Kim, HyeRan Kim, ChulWook Ahn, Jun Cheul Cho, Hye Sun Int J Mol Sci Article Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. We compared heat-responsive gene expression between a heat-tolerant cabbage line (HTCL), “HO”, and a heat-sensitive cabbage line (HSCL), “JK”, by Genechip assay. Expression levels of specific heat stress-related genes were increased in response to high-temperature stress, according to Genechip assays. We performed quantitative RT-PCR (qRT-PCR) to compare expression levels of these heat stress-related genes in four HTCLs and four HSCLs. Transcript levels for heat shock protein BoHsp70 and transcription factor BoGRAS (SCL13) were more strongly expressed only in all HTCLs compared to all HSCLs, showing much lower level expressions at the young plant stage under heat stress (HS). Thus, we suggest that expression levels of these genes may be early selection markers for HTCLs in cabbage breeding. In addition, several genes that are involved in the secondary metabolite pathway were differentially regulated in HTCL and HSCL exposed to heat stress. Molecular Diversity Preservation International (MDPI) 2013-06-04 /pmc/articles/PMC3709761/ /pubmed/23736694 http://dx.doi.org/10.3390/ijms140611871 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Park, Hyun Ji
Jung, Won Yong
Lee, Sang Sook
Song, Jun Ho
Kwon, Suk-Yoon
Kim, HyeRan
Kim, ChulWook
Ahn, Jun Cheul
Cho, Hye Sun
Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)
title Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)
title_full Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)
title_fullStr Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)
title_full_unstemmed Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)
title_short Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)
title_sort use of heat stress responsive gene expression levels for early selection of heat tolerant cabbage (brassica oleracea l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709761/
https://www.ncbi.nlm.nih.gov/pubmed/23736694
http://dx.doi.org/10.3390/ijms140611871
work_keys_str_mv AT parkhyunji useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT jungwonyong useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT leesangsook useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT songjunho useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT kwonsukyoon useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT kimhyeran useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT kimchulwook useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT ahnjuncheul useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal
AT chohyesun useofheatstressresponsivegeneexpressionlevelsforearlyselectionofheattolerantcabbagebrassicaoleraceal