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Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder
The orphan GPR87 has recently been matched with its ligand LPA, which is a lipid mediator with multiple physiological functions, including cancer cell proliferation. This study aimed to clarify the role of GPR87 in urothelial carcinoma of the bladder. GPR87 expression was assessed in seven human bla...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709790/ https://www.ncbi.nlm.nih.gov/pubmed/23752273 http://dx.doi.org/10.3390/ijms140612367 |
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author | Okazoe, Homare Zhang, Xia Liu, Dage Shibuya, Shinsuke Ueda, Nobufumi Sugimoto, Mikio Kakehi, Yoshiyuki |
author_facet | Okazoe, Homare Zhang, Xia Liu, Dage Shibuya, Shinsuke Ueda, Nobufumi Sugimoto, Mikio Kakehi, Yoshiyuki |
author_sort | Okazoe, Homare |
collection | PubMed |
description | The orphan GPR87 has recently been matched with its ligand LPA, which is a lipid mediator with multiple physiological functions, including cancer cell proliferation. This study aimed to clarify the role of GPR87 in urothelial carcinoma of the bladder. GPR87 expression was assessed in seven human bladder cancer cell lines. A replication-deficient recombinant adenoviral vector expressing shRNA targeting GPR87 (Ad-shGPR87), was constructed. Gene silencing was carried out using Ad-shGPR87. Immunohistochemical analysis was performed for transurethral resection of bladder tumor samples from 71 patients with non-muscle-invasive bladder cancer. We observed GPR87 expression in five of the seven cell lines, and silencing GPR87 gene expression significantly reduced cell viability. GPR87 expression was positive in 38 (54%) of 71 tumors. Ki-67 index was associated with positive GPR87 staining status (p < 0.0001). Patients with GPR87-positive tumors had shorter intravesical recurrence-free survival than those with GPR87-negative tumors (p = 0.010). Multivariate analysis revealed that GPR87 staining status was an independent prognostic parameter for intravesical recurrence (p = 0.041). Progression from non-muscle-invasive to muscle-invasive tumor was more frequently observed in patients with GPR87-positive tumors, although this trend did not reach statistical significance (p = 0.056). These results warrant further prospective studies to clarify the role of GPR87 expression in intravesical recurrence and progression in bladder cancer. |
format | Online Article Text |
id | pubmed-3709790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-37097902013-07-12 Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder Okazoe, Homare Zhang, Xia Liu, Dage Shibuya, Shinsuke Ueda, Nobufumi Sugimoto, Mikio Kakehi, Yoshiyuki Int J Mol Sci Article The orphan GPR87 has recently been matched with its ligand LPA, which is a lipid mediator with multiple physiological functions, including cancer cell proliferation. This study aimed to clarify the role of GPR87 in urothelial carcinoma of the bladder. GPR87 expression was assessed in seven human bladder cancer cell lines. A replication-deficient recombinant adenoviral vector expressing shRNA targeting GPR87 (Ad-shGPR87), was constructed. Gene silencing was carried out using Ad-shGPR87. Immunohistochemical analysis was performed for transurethral resection of bladder tumor samples from 71 patients with non-muscle-invasive bladder cancer. We observed GPR87 expression in five of the seven cell lines, and silencing GPR87 gene expression significantly reduced cell viability. GPR87 expression was positive in 38 (54%) of 71 tumors. Ki-67 index was associated with positive GPR87 staining status (p < 0.0001). Patients with GPR87-positive tumors had shorter intravesical recurrence-free survival than those with GPR87-negative tumors (p = 0.010). Multivariate analysis revealed that GPR87 staining status was an independent prognostic parameter for intravesical recurrence (p = 0.041). Progression from non-muscle-invasive to muscle-invasive tumor was more frequently observed in patients with GPR87-positive tumors, although this trend did not reach statistical significance (p = 0.056). These results warrant further prospective studies to clarify the role of GPR87 expression in intravesical recurrence and progression in bladder cancer. Molecular Diversity Preservation International (MDPI) 2013-06-10 /pmc/articles/PMC3709790/ /pubmed/23752273 http://dx.doi.org/10.3390/ijms140612367 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Okazoe, Homare Zhang, Xia Liu, Dage Shibuya, Shinsuke Ueda, Nobufumi Sugimoto, Mikio Kakehi, Yoshiyuki Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder |
title | Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder |
title_full | Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder |
title_fullStr | Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder |
title_full_unstemmed | Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder |
title_short | Expression and Role of GPR87 in Urothelial Carcinoma of the Bladder |
title_sort | expression and role of gpr87 in urothelial carcinoma of the bladder |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709790/ https://www.ncbi.nlm.nih.gov/pubmed/23752273 http://dx.doi.org/10.3390/ijms140612367 |
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