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An update on the molecular genetics toolbox for staphylococci
Staphylococci are Gram-positive spherical bacteria of enormous clinical and biotechnological relevance. Staphylococcus aureus has been extensively studied as a model pathogen. A plethora of methods and molecular tools has been developed for genetic modification of at least ten different staphylococc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for General Microbiology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709823/ https://www.ncbi.nlm.nih.gov/pubmed/23378573 http://dx.doi.org/10.1099/mic.0.061705-0 |
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author | Prax, Marcel Lee, Chia Y. Bertram, Ralph |
author_facet | Prax, Marcel Lee, Chia Y. Bertram, Ralph |
author_sort | Prax, Marcel |
collection | PubMed |
description | Staphylococci are Gram-positive spherical bacteria of enormous clinical and biotechnological relevance. Staphylococcus aureus has been extensively studied as a model pathogen. A plethora of methods and molecular tools has been developed for genetic modification of at least ten different staphylococcal species to date. Here we review recent developments of various genetic tools and molecular methods for staphylococcal research, which include reporter systems and vectors for controllable gene expression, gene inactivation, gene essentiality testing, chromosomal integration and transposon delivery. It is furthermore illustrated how mutant strain construction by homologous or site-specific recombination benefits from sophisticated counterselection methods. The underlying genetic components have been shown to operate in wild-type staphylococci or modified chassis strains. Finally, possible future developments in the field of applied Staphylococcus genetics are highlighted. |
format | Online Article Text |
id | pubmed-3709823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Society for General Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-37098232013-07-24 An update on the molecular genetics toolbox for staphylococci Prax, Marcel Lee, Chia Y. Bertram, Ralph Microbiology (Reading) Review Staphylococci are Gram-positive spherical bacteria of enormous clinical and biotechnological relevance. Staphylococcus aureus has been extensively studied as a model pathogen. A plethora of methods and molecular tools has been developed for genetic modification of at least ten different staphylococcal species to date. Here we review recent developments of various genetic tools and molecular methods for staphylococcal research, which include reporter systems and vectors for controllable gene expression, gene inactivation, gene essentiality testing, chromosomal integration and transposon delivery. It is furthermore illustrated how mutant strain construction by homologous or site-specific recombination benefits from sophisticated counterselection methods. The underlying genetic components have been shown to operate in wild-type staphylococci or modified chassis strains. Finally, possible future developments in the field of applied Staphylococcus genetics are highlighted. Society for General Microbiology 2013-03 /pmc/articles/PMC3709823/ /pubmed/23378573 http://dx.doi.org/10.1099/mic.0.061705-0 Text en © 2013 SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Prax, Marcel Lee, Chia Y. Bertram, Ralph An update on the molecular genetics toolbox for staphylococci |
title | An update on the molecular genetics toolbox for staphylococci |
title_full | An update on the molecular genetics toolbox for staphylococci |
title_fullStr | An update on the molecular genetics toolbox for staphylococci |
title_full_unstemmed | An update on the molecular genetics toolbox for staphylococci |
title_short | An update on the molecular genetics toolbox for staphylococci |
title_sort | update on the molecular genetics toolbox for staphylococci |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709823/ https://www.ncbi.nlm.nih.gov/pubmed/23378573 http://dx.doi.org/10.1099/mic.0.061705-0 |
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