Cargando…

Overexpression of membrane proteins in mammalian cells for structural studies

The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny propor...

Descripción completa

Detalles Bibliográficos
Autores principales: Andréll, Juni, Tate, Christopher G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa UK, Ltd. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709882/
https://www.ncbi.nlm.nih.gov/pubmed/22963530
http://dx.doi.org/10.3109/09687688.2012.703703
_version_ 1782276813037240320
author Andréll, Juni
Tate, Christopher G.
author_facet Andréll, Juni
Tate, Christopher G.
author_sort Andréll, Juni
collection PubMed
description The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total number of membrane proteins encoded by a mammalian genome. In addition, the structures determined to date are of the most tractable membrane proteins, i.e., those that are expressed functionally and to high levels in yeast or in insect cells using the baculovirus expression system. However, some membrane proteins that are expressed inefficiently in these systems can be produced at sufficiently high levels in mammalian cells to allow structure determination. Mammalian expression systems are an under-used resource in structural biology and represent an effective way to produce fully functional membrane proteins for structural studies. This review will discuss examples of vertebrate membrane protein overexpression in mammalian cells using a variety of viral, constitutive or inducible expression systems.
format Online
Article
Text
id pubmed-3709882
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Informa UK, Ltd.
record_format MEDLINE/PubMed
spelling pubmed-37098822013-07-16 Overexpression of membrane proteins in mammalian cells for structural studies Andréll, Juni Tate, Christopher G. Mol Membr Biol Article The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total number of membrane proteins encoded by a mammalian genome. In addition, the structures determined to date are of the most tractable membrane proteins, i.e., those that are expressed functionally and to high levels in yeast or in insect cells using the baculovirus expression system. However, some membrane proteins that are expressed inefficiently in these systems can be produced at sufficiently high levels in mammalian cells to allow structure determination. Mammalian expression systems are an under-used resource in structural biology and represent an effective way to produce fully functional membrane proteins for structural studies. This review will discuss examples of vertebrate membrane protein overexpression in mammalian cells using a variety of viral, constitutive or inducible expression systems. Informa UK, Ltd. 2013-02 2012-09-11 /pmc/articles/PMC3709882/ /pubmed/22963530 http://dx.doi.org/10.3109/09687688.2012.703703 Text en © Informa UK, Ltd. http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.
spellingShingle Article
Andréll, Juni
Tate, Christopher G.
Overexpression of membrane proteins in mammalian cells for structural studies
title Overexpression of membrane proteins in mammalian cells for structural studies
title_full Overexpression of membrane proteins in mammalian cells for structural studies
title_fullStr Overexpression of membrane proteins in mammalian cells for structural studies
title_full_unstemmed Overexpression of membrane proteins in mammalian cells for structural studies
title_short Overexpression of membrane proteins in mammalian cells for structural studies
title_sort overexpression of membrane proteins in mammalian cells for structural studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709882/
https://www.ncbi.nlm.nih.gov/pubmed/22963530
http://dx.doi.org/10.3109/09687688.2012.703703
work_keys_str_mv AT andrelljuni overexpressionofmembraneproteinsinmammaliancellsforstructuralstudies
AT tatechristopherg overexpressionofmembraneproteinsinmammaliancellsforstructuralstudies