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Developmental potential of human oocytes matured in vitro followed by vitrification and activation
BACKGROUND: Oocyte in vitro maturation (IVM) and cryopreservation at the time of routine ovarian tissue freezing may be offered to cancer patients as an additional option for fertility preservation. This study aimed to investigate the developmental capacity of oocytes isolated from unstimulated ovar...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710234/ https://www.ncbi.nlm.nih.gov/pubmed/23597104 http://dx.doi.org/10.1186/1757-2215-6-30 |
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author | Imesch, Patrick Scheiner, David Xie, Min Fink, Daniel Macas, Erwin Dubey, Raghvendra Imthurn, Bruno |
author_facet | Imesch, Patrick Scheiner, David Xie, Min Fink, Daniel Macas, Erwin Dubey, Raghvendra Imthurn, Bruno |
author_sort | Imesch, Patrick |
collection | PubMed |
description | BACKGROUND: Oocyte in vitro maturation (IVM) and cryopreservation at the time of routine ovarian tissue freezing may be offered to cancer patients as an additional option for fertility preservation. This study aimed to investigate the developmental capacity of oocytes isolated from unstimulated ovaries. METHODS: Immature oocytes (n = 63) from seven consenting premenopausal patients were analysed. Oocytes were collected during routine laparoscopic examination with biopsy of an ovary (cystic adnexal mass, n = 3; cervical adenocarcinoma, n = 2) or oophorectomy (sex reassignment surgery, n = 2) without previous stimulation of the ovaries. The stage of the patient’s menstrual cycle was not considered. Oocytes in all visible antral follicles were aspirated from ovaries, cultured in IVM medium and vitrified at the MII stage before being kept in liquid nitrogen for at least one month. After warming, oocytes were subjected to parthenogenetic activation by chemical stimulus. Their further development was recorded at intervals of 24 hours for up to 6 days of culture. RESULTS: 61.9% of oocytes matured in vitro within 48 hours. The survival rate after vitrification and warming was 61.5%. A total of 75% of surviving oocytes were able to respond to artificial activation, 44.4% of the parthenotes developed to early embryonic stage. However, only 1 in 18 (5.6%) of the resulting embryos reached blastocyst stage. CONCLUSIONS: Oocytes matured in vitro from unstimulated ovaries seem to have limited developmental potential after cryopreservation and artificial activation. Although the outcome of IVM for non-stimulated oocytes is poor, it is currently the only chance besides cryopreservation of ovarian tissue for women for whom ovarian stimulation is not possible due to life circumstances. Based on our preliminary results, we suggest that the use of cryopreserved ovaries for fertility preservation in women with cancer warrants further investigation. |
format | Online Article Text |
id | pubmed-3710234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-37102342013-07-13 Developmental potential of human oocytes matured in vitro followed by vitrification and activation Imesch, Patrick Scheiner, David Xie, Min Fink, Daniel Macas, Erwin Dubey, Raghvendra Imthurn, Bruno J Ovarian Res Research BACKGROUND: Oocyte in vitro maturation (IVM) and cryopreservation at the time of routine ovarian tissue freezing may be offered to cancer patients as an additional option for fertility preservation. This study aimed to investigate the developmental capacity of oocytes isolated from unstimulated ovaries. METHODS: Immature oocytes (n = 63) from seven consenting premenopausal patients were analysed. Oocytes were collected during routine laparoscopic examination with biopsy of an ovary (cystic adnexal mass, n = 3; cervical adenocarcinoma, n = 2) or oophorectomy (sex reassignment surgery, n = 2) without previous stimulation of the ovaries. The stage of the patient’s menstrual cycle was not considered. Oocytes in all visible antral follicles were aspirated from ovaries, cultured in IVM medium and vitrified at the MII stage before being kept in liquid nitrogen for at least one month. After warming, oocytes were subjected to parthenogenetic activation by chemical stimulus. Their further development was recorded at intervals of 24 hours for up to 6 days of culture. RESULTS: 61.9% of oocytes matured in vitro within 48 hours. The survival rate after vitrification and warming was 61.5%. A total of 75% of surviving oocytes were able to respond to artificial activation, 44.4% of the parthenotes developed to early embryonic stage. However, only 1 in 18 (5.6%) of the resulting embryos reached blastocyst stage. CONCLUSIONS: Oocytes matured in vitro from unstimulated ovaries seem to have limited developmental potential after cryopreservation and artificial activation. Although the outcome of IVM for non-stimulated oocytes is poor, it is currently the only chance besides cryopreservation of ovarian tissue for women for whom ovarian stimulation is not possible due to life circumstances. Based on our preliminary results, we suggest that the use of cryopreserved ovaries for fertility preservation in women with cancer warrants further investigation. BioMed Central 2013-04-18 /pmc/articles/PMC3710234/ /pubmed/23597104 http://dx.doi.org/10.1186/1757-2215-6-30 Text en Copyright © 2013 Imesch et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Imesch, Patrick Scheiner, David Xie, Min Fink, Daniel Macas, Erwin Dubey, Raghvendra Imthurn, Bruno Developmental potential of human oocytes matured in vitro followed by vitrification and activation |
title | Developmental potential of human oocytes matured in vitro followed by vitrification and activation |
title_full | Developmental potential of human oocytes matured in vitro followed by vitrification and activation |
title_fullStr | Developmental potential of human oocytes matured in vitro followed by vitrification and activation |
title_full_unstemmed | Developmental potential of human oocytes matured in vitro followed by vitrification and activation |
title_short | Developmental potential of human oocytes matured in vitro followed by vitrification and activation |
title_sort | developmental potential of human oocytes matured in vitro followed by vitrification and activation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710234/ https://www.ncbi.nlm.nih.gov/pubmed/23597104 http://dx.doi.org/10.1186/1757-2215-6-30 |
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