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Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences

BACKGROUND: Dendropsophus is a monophyletic anuran genus with a diploid number of 30 chromosomes as an important synapomorphy. However, the internal phylogenetic relationships of this genus are poorly understood. Interestingly, an intriguing interspecific variation in the telocentric chromosome numb...

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Autores principales: Medeiros, Lilian Ricco, Lourenço, Luciana Bolsoni, Rossa-Feres, Denise Cerqueira, Lima, Albertina Pimentel, Andrade, Gilda Vasconcellos, Giaretta, Ariovaldo Antonio, Egito, Gabriel Toselli Barbosa Tabosa, Recco-Pimentel, Shirlei Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710474/
https://www.ncbi.nlm.nih.gov/pubmed/23822759
http://dx.doi.org/10.1186/1471-2156-14-59
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author Medeiros, Lilian Ricco
Lourenço, Luciana Bolsoni
Rossa-Feres, Denise Cerqueira
Lima, Albertina Pimentel
Andrade, Gilda Vasconcellos
Giaretta, Ariovaldo Antonio
Egito, Gabriel Toselli Barbosa Tabosa
Recco-Pimentel, Shirlei Maria
author_facet Medeiros, Lilian Ricco
Lourenço, Luciana Bolsoni
Rossa-Feres, Denise Cerqueira
Lima, Albertina Pimentel
Andrade, Gilda Vasconcellos
Giaretta, Ariovaldo Antonio
Egito, Gabriel Toselli Barbosa Tabosa
Recco-Pimentel, Shirlei Maria
author_sort Medeiros, Lilian Ricco
collection PubMed
description BACKGROUND: Dendropsophus is a monophyletic anuran genus with a diploid number of 30 chromosomes as an important synapomorphy. However, the internal phylogenetic relationships of this genus are poorly understood. Interestingly, an intriguing interspecific variation in the telocentric chromosome number has been useful in species identification. To address certain uncertainties related to one of the species groups of Dendropsophus, the D. microcephalus group, we carried out a cytogenetic analysis combined with phylogenetic inferences based on mitochondrial sequences, which aimed to aid in the analysis of chromosomal characters. Populations of Dendropsophus nanus, Dendropsophus walfordi, Dendropsophus sanborni, Dendropsophus jimi and Dendropsophus elianeae, ranging from the extreme south to the north of Brazil, were cytogenetically compared. A mitochondrial region of the ribosomal 12S gene from these populations, as well as from 30 other species of Dendropsophus, was used for the phylogenetic inferences. Phylogenetic relationships were inferred using maximum parsimony and Bayesian analyses. RESULTS: The species D. nanus and D. walfordi exhibited identical karyotypes (2n = 30; FN = 52), with four pairs of telocentric chromosomes and a NOR located on metacentric chromosome pair 13. In all of the phylogenetic hypotheses, the paraphyly of D. nanus and D. walfordi was inferred. D. sanborni from Botucatu-SP and Torres-RS showed the same karyotype as D. jimi, with 5 pairs of telocentric chromosomes (2n = 30; FN = 50) and a terminal NOR in the long arm of the telocentric chromosome pair 12. Despite their karyotypic similarity, these species were not found to compose a monophyletic group. Finally, the phylogenetic and cytogenetic analyses did not cluster the specimens of D. elianeae according to their geographical occurrence or recognized morphotypes. CONCLUSIONS: We suggest that a taxonomic revision of the taxa D. nanus and D. walfordi is quite necessary. We also observe that the number of telocentric chromosomes is useful to distinguish among valid species in some cases, although it is unchanged in species that are not necessarily closely related phylogenetically. Therefore, inferences based on this chromosomal character must be made with caution; a proper evolutionary analysis of the karyotypic variation in Dendropsophus depends on further characterization of the telocentric chromosomes found in this group.
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spelling pubmed-37104742013-07-14 Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences Medeiros, Lilian Ricco Lourenço, Luciana Bolsoni Rossa-Feres, Denise Cerqueira Lima, Albertina Pimentel Andrade, Gilda Vasconcellos Giaretta, Ariovaldo Antonio Egito, Gabriel Toselli Barbosa Tabosa Recco-Pimentel, Shirlei Maria BMC Genet Research Article BACKGROUND: Dendropsophus is a monophyletic anuran genus with a diploid number of 30 chromosomes as an important synapomorphy. However, the internal phylogenetic relationships of this genus are poorly understood. Interestingly, an intriguing interspecific variation in the telocentric chromosome number has been useful in species identification. To address certain uncertainties related to one of the species groups of Dendropsophus, the D. microcephalus group, we carried out a cytogenetic analysis combined with phylogenetic inferences based on mitochondrial sequences, which aimed to aid in the analysis of chromosomal characters. Populations of Dendropsophus nanus, Dendropsophus walfordi, Dendropsophus sanborni, Dendropsophus jimi and Dendropsophus elianeae, ranging from the extreme south to the north of Brazil, were cytogenetically compared. A mitochondrial region of the ribosomal 12S gene from these populations, as well as from 30 other species of Dendropsophus, was used for the phylogenetic inferences. Phylogenetic relationships were inferred using maximum parsimony and Bayesian analyses. RESULTS: The species D. nanus and D. walfordi exhibited identical karyotypes (2n = 30; FN = 52), with four pairs of telocentric chromosomes and a NOR located on metacentric chromosome pair 13. In all of the phylogenetic hypotheses, the paraphyly of D. nanus and D. walfordi was inferred. D. sanborni from Botucatu-SP and Torres-RS showed the same karyotype as D. jimi, with 5 pairs of telocentric chromosomes (2n = 30; FN = 50) and a terminal NOR in the long arm of the telocentric chromosome pair 12. Despite their karyotypic similarity, these species were not found to compose a monophyletic group. Finally, the phylogenetic and cytogenetic analyses did not cluster the specimens of D. elianeae according to their geographical occurrence or recognized morphotypes. CONCLUSIONS: We suggest that a taxonomic revision of the taxa D. nanus and D. walfordi is quite necessary. We also observe that the number of telocentric chromosomes is useful to distinguish among valid species in some cases, although it is unchanged in species that are not necessarily closely related phylogenetically. Therefore, inferences based on this chromosomal character must be made with caution; a proper evolutionary analysis of the karyotypic variation in Dendropsophus depends on further characterization of the telocentric chromosomes found in this group. BioMed Central 2013-07-03 /pmc/articles/PMC3710474/ /pubmed/23822759 http://dx.doi.org/10.1186/1471-2156-14-59 Text en Copyright © 2013 Medeiros et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Medeiros, Lilian Ricco
Lourenço, Luciana Bolsoni
Rossa-Feres, Denise Cerqueira
Lima, Albertina Pimentel
Andrade, Gilda Vasconcellos
Giaretta, Ariovaldo Antonio
Egito, Gabriel Toselli Barbosa Tabosa
Recco-Pimentel, Shirlei Maria
Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences
title Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences
title_full Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences
title_fullStr Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences
title_full_unstemmed Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences
title_short Comparative cytogenetic analysis of some species of the Dendropsophus microcephalus group (Anura, Hylidae) in the light of phylogenetic inferences
title_sort comparative cytogenetic analysis of some species of the dendropsophus microcephalus group (anura, hylidae) in the light of phylogenetic inferences
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710474/
https://www.ncbi.nlm.nih.gov/pubmed/23822759
http://dx.doi.org/10.1186/1471-2156-14-59
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