DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions

The expression of adenovirus late genes is shown to require viral DNA replication, but its mechanism remains elusive. Here we found that knockdown of CTCF suppresses viral DNA replication as well as late, but not early, gene expression. Chromatin immunoprecipitation assays indicated that CTCF binds...

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Autores principales: Komatsu, Tetsuro, Sekiya, Takeshi, Nagata, Kyosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711053/
https://www.ncbi.nlm.nih.gov/pubmed/23851926
http://dx.doi.org/10.1038/srep02187
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author Komatsu, Tetsuro
Sekiya, Takeshi
Nagata, Kyosuke
author_facet Komatsu, Tetsuro
Sekiya, Takeshi
Nagata, Kyosuke
author_sort Komatsu, Tetsuro
collection PubMed
description The expression of adenovirus late genes is shown to require viral DNA replication, but its mechanism remains elusive. Here we found that knockdown of CTCF suppresses viral DNA replication as well as late, but not early, gene expression. Chromatin immunoprecipitation assays indicated that CTCF binds to viral chromatin depending on viral DNA replication. These findings depict CTCF as a critical regulator for adenovirus genome functions in late phases of infection.
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spelling pubmed-37110532013-07-15 DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions Komatsu, Tetsuro Sekiya, Takeshi Nagata, Kyosuke Sci Rep Article The expression of adenovirus late genes is shown to require viral DNA replication, but its mechanism remains elusive. Here we found that knockdown of CTCF suppresses viral DNA replication as well as late, but not early, gene expression. Chromatin immunoprecipitation assays indicated that CTCF binds to viral chromatin depending on viral DNA replication. These findings depict CTCF as a critical regulator for adenovirus genome functions in late phases of infection. Nature Publishing Group 2013-07-15 /pmc/articles/PMC3711053/ /pubmed/23851926 http://dx.doi.org/10.1038/srep02187 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Komatsu, Tetsuro
Sekiya, Takeshi
Nagata, Kyosuke
DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions
title DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions
title_full DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions
title_fullStr DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions
title_full_unstemmed DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions
title_short DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions
title_sort dna replication-dependent binding of ctcf plays a critical role in adenovirus genome functions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711053/
https://www.ncbi.nlm.nih.gov/pubmed/23851926
http://dx.doi.org/10.1038/srep02187
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AT sekiyatakeshi dnareplicationdependentbindingofctcfplaysacriticalroleinadenovirusgenomefunctions
AT nagatakyosuke dnareplicationdependentbindingofctcfplaysacriticalroleinadenovirusgenomefunctions

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