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Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns

The generation of micro- and nano-topography similar to those found in the extra cellular matrix of three-dimensional tissues is one technique used to recapitulate the cell-tissue physiology found in the native tissues. Despite the fact that ample studies have been conducted on the physiological sig...

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Autores principales: Hatano, Rachel, Mercurio, Kevin, Luna, Jesus Isaac, Glaser, Drew E, Leppert, Valerie J, McCloskey, Kara E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711924/
https://www.ncbi.nlm.nih.gov/pubmed/23819656
http://dx.doi.org/10.1186/1754-1611-7-18
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author Hatano, Rachel
Mercurio, Kevin
Luna, Jesus Isaac
Glaser, Drew E
Leppert, Valerie J
McCloskey, Kara E
author_facet Hatano, Rachel
Mercurio, Kevin
Luna, Jesus Isaac
Glaser, Drew E
Leppert, Valerie J
McCloskey, Kara E
author_sort Hatano, Rachel
collection PubMed
description The generation of micro- and nano-topography similar to those found in the extra cellular matrix of three-dimensional tissues is one technique used to recapitulate the cell-tissue physiology found in the native tissues. Despite the fact that ample studies have been conducted on the physiological significance of endothelial cells alignment parallel to shear stress, as this is the normal physiologic arrangement for healthy arterial EC, very few studies have examined the use of topographical signals to initiate endothelial cell alignment. Here, we have examined the ability for our mouse embryonic stem cell-derived endothelial cells (ESC-EC) to align on various microchip topographical systems. Briefly, we generated metal molds with ‘wrinkled’ topography using 1) 15 nm and 2) 30 nm of gold coating on the pre-strained polystryene (PS) sheets. After thermal-induced shrinkage of the PS sheets, polydimethylsiloxane (PDMS) microchips were then generated from the wrinkled molds. Using similar Shrink™-based technology, 3) larger selectively crazed acetone-etched lines in the PS sheets, and 4) fully crazed acetone-treated PS sheets of stochastic topographical morphology were also generated. The 15 nm and 30 nm gold coating generated ‘wrinkles’ of uniaxial anisotropic channels at nano-scaled widths while the crazing generated micron-sized channels. The ESC-EC were able to respond and align on the 320 nm, 510 nm, and the acetone-etched 10.5 μm channels, but not on the fully ‘crazed’ topographies. Moreover, the ESC-EC aligned most robustly on the wrinkles, and preferentially to ridge edges on the 10.5 μm-sized channels. The ability to robustly align EC on topographical surfaces enables a variety of controlled physiological studies of EC-EC and EC-ECM contact guidance, as well as having potential applications for the rapid endothelialization of stents and vascular grafts.
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spelling pubmed-37119242013-07-16 Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns Hatano, Rachel Mercurio, Kevin Luna, Jesus Isaac Glaser, Drew E Leppert, Valerie J McCloskey, Kara E J Biol Eng Research The generation of micro- and nano-topography similar to those found in the extra cellular matrix of three-dimensional tissues is one technique used to recapitulate the cell-tissue physiology found in the native tissues. Despite the fact that ample studies have been conducted on the physiological significance of endothelial cells alignment parallel to shear stress, as this is the normal physiologic arrangement for healthy arterial EC, very few studies have examined the use of topographical signals to initiate endothelial cell alignment. Here, we have examined the ability for our mouse embryonic stem cell-derived endothelial cells (ESC-EC) to align on various microchip topographical systems. Briefly, we generated metal molds with ‘wrinkled’ topography using 1) 15 nm and 2) 30 nm of gold coating on the pre-strained polystryene (PS) sheets. After thermal-induced shrinkage of the PS sheets, polydimethylsiloxane (PDMS) microchips were then generated from the wrinkled molds. Using similar Shrink™-based technology, 3) larger selectively crazed acetone-etched lines in the PS sheets, and 4) fully crazed acetone-treated PS sheets of stochastic topographical morphology were also generated. The 15 nm and 30 nm gold coating generated ‘wrinkles’ of uniaxial anisotropic channels at nano-scaled widths while the crazing generated micron-sized channels. The ESC-EC were able to respond and align on the 320 nm, 510 nm, and the acetone-etched 10.5 μm channels, but not on the fully ‘crazed’ topographies. Moreover, the ESC-EC aligned most robustly on the wrinkles, and preferentially to ridge edges on the 10.5 μm-sized channels. The ability to robustly align EC on topographical surfaces enables a variety of controlled physiological studies of EC-EC and EC-ECM contact guidance, as well as having potential applications for the rapid endothelialization of stents and vascular grafts. BioMed Central 2013-07-02 /pmc/articles/PMC3711924/ /pubmed/23819656 http://dx.doi.org/10.1186/1754-1611-7-18 Text en Copyright © 2013 Hatano et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hatano, Rachel
Mercurio, Kevin
Luna, Jesus Isaac
Glaser, Drew E
Leppert, Valerie J
McCloskey, Kara E
Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns
title Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns
title_full Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns
title_fullStr Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns
title_full_unstemmed Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns
title_short Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns
title_sort endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711924/
https://www.ncbi.nlm.nih.gov/pubmed/23819656
http://dx.doi.org/10.1186/1754-1611-7-18
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