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Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin
INTRODUCTION: Posterior capsule rupture causes instant vitreous contamination, resulting in endophthalmitis. However, transfer of intracameral moxifloxacin (MFLX) to the vitreous has not been examined in detail. We investigated vitreous antibiotic concentrations following intracameral MFLX in both r...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711953/ https://www.ncbi.nlm.nih.gov/pubmed/23874080 http://dx.doi.org/10.2147/OPTH.S47629 |
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author | Matsuura, Kazuki Inoue, Yoshitsugu Sasaki, Shin-ichi Hata, Yoshio Ohmura, Nami Gotou, Takahiro |
author_facet | Matsuura, Kazuki Inoue, Yoshitsugu Sasaki, Shin-ichi Hata, Yoshio Ohmura, Nami Gotou, Takahiro |
author_sort | Matsuura, Kazuki |
collection | PubMed |
description | INTRODUCTION: Posterior capsule rupture causes instant vitreous contamination, resulting in endophthalmitis. However, transfer of intracameral moxifloxacin (MFLX) to the vitreous has not been examined in detail. We investigated vitreous antibiotic concentrations following intracameral MFLX in both ruptured and intact posterior capsular eyes. METHODS: Experiment 1: Intraocular lenses were inserted into 21 extracted porcine eyes by one of the following three methods: (1) Irrigation: Throughout surgery, 33-fold diluted MFLX irrigation solution (150 μg/mL) was used; (2) Bag and chamber flushing: After surgery, the anterior chamber and area behind the intraocular lenses were irrigated with 30-fold diluted MFLX (167 μg/mL) using a 5 mL syringe; (3) Simple injection: Tenfold diluted MFLX (50 μg in 0.1 mL) was injected intracamerally at the conclusion of surgery. The eyeballs were frozen and the anterior, central, and posterior portions of the vitreous were cubed. After defrosting, concentrations were measured using high-performance liquid chromatography. Experiment 2: The same procedure was conducted for 18 eyes in which the posterior capsule had been ruptured. RESULTS: Experiment 1: Transfer of intracameral MFLX to the anterior vitreous was approximately 1% (1.56–2.20 μg/mL) regardless of the administration method. Experiment 2: MFLX reached a high concentration in the vitreous with irrigation solution administration (maximum 30.22 μg/mL). The concentrations reached by simple injection or flushing were significantly less than those obtained by irrigation. CONCLUSION: With an intact posterior capsule, intracameral MFLX exhibited limited effects on vitreous concentration. Despite the fact that the risk of infection clearly increases in cases of ruptured capsule, no special infection prevention protocol has been proposed. It was confirmed that irrigation solution caused vitreous contamination in ruptured eyes within only a short irrigation time. In this case, intracameral administration did not necessarily achieve preventive concentrations for endophthalmitis, but it appears that an effective drug concentration can be achieved in the vitreous by the administration of irrigation solution. |
format | Online Article Text |
id | pubmed-3711953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-37119532013-07-19 Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin Matsuura, Kazuki Inoue, Yoshitsugu Sasaki, Shin-ichi Hata, Yoshio Ohmura, Nami Gotou, Takahiro Clin Ophthalmol Original Research INTRODUCTION: Posterior capsule rupture causes instant vitreous contamination, resulting in endophthalmitis. However, transfer of intracameral moxifloxacin (MFLX) to the vitreous has not been examined in detail. We investigated vitreous antibiotic concentrations following intracameral MFLX in both ruptured and intact posterior capsular eyes. METHODS: Experiment 1: Intraocular lenses were inserted into 21 extracted porcine eyes by one of the following three methods: (1) Irrigation: Throughout surgery, 33-fold diluted MFLX irrigation solution (150 μg/mL) was used; (2) Bag and chamber flushing: After surgery, the anterior chamber and area behind the intraocular lenses were irrigated with 30-fold diluted MFLX (167 μg/mL) using a 5 mL syringe; (3) Simple injection: Tenfold diluted MFLX (50 μg in 0.1 mL) was injected intracamerally at the conclusion of surgery. The eyeballs were frozen and the anterior, central, and posterior portions of the vitreous were cubed. After defrosting, concentrations were measured using high-performance liquid chromatography. Experiment 2: The same procedure was conducted for 18 eyes in which the posterior capsule had been ruptured. RESULTS: Experiment 1: Transfer of intracameral MFLX to the anterior vitreous was approximately 1% (1.56–2.20 μg/mL) regardless of the administration method. Experiment 2: MFLX reached a high concentration in the vitreous with irrigation solution administration (maximum 30.22 μg/mL). The concentrations reached by simple injection or flushing were significantly less than those obtained by irrigation. CONCLUSION: With an intact posterior capsule, intracameral MFLX exhibited limited effects on vitreous concentration. Despite the fact that the risk of infection clearly increases in cases of ruptured capsule, no special infection prevention protocol has been proposed. It was confirmed that irrigation solution caused vitreous contamination in ruptured eyes within only a short irrigation time. In this case, intracameral administration did not necessarily achieve preventive concentrations for endophthalmitis, but it appears that an effective drug concentration can be achieved in the vitreous by the administration of irrigation solution. Dove Medical Press 2013 2013-07-09 /pmc/articles/PMC3711953/ /pubmed/23874080 http://dx.doi.org/10.2147/OPTH.S47629 Text en © 2013 Matsuura et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Matsuura, Kazuki Inoue, Yoshitsugu Sasaki, Shin-ichi Hata, Yoshio Ohmura, Nami Gotou, Takahiro Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin |
title | Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin |
title_full | Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin |
title_fullStr | Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin |
title_full_unstemmed | Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin |
title_short | Assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin |
title_sort | assessment of vitreous drug concentration in the porcine eye following intracameral injection or irrigation with moxifloxacin |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711953/ https://www.ncbi.nlm.nih.gov/pubmed/23874080 http://dx.doi.org/10.2147/OPTH.S47629 |
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