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Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep
According to increase of travel, the cases of imported echinococcosis have been increasing in Korea. The present study was undertaken to develop a serodiagnostic system for echinococcosis in Korea. For diagnosis of echinococcosis, the fluid of Echinococcus granulosus hydatid cysts was collected from...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Parasitology and Tropical Medicine
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712105/ https://www.ncbi.nlm.nih.gov/pubmed/23864742 http://dx.doi.org/10.3347/kjp.2013.51.3.313 |
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author | Jin, Yan Anvarov, Khikmat Khajibaev, Abdukhakim Hong, Samin Hong, Sung-Tae |
author_facet | Jin, Yan Anvarov, Khikmat Khajibaev, Abdukhakim Hong, Samin Hong, Sung-Tae |
author_sort | Jin, Yan |
collection | PubMed |
description | According to increase of travel, the cases of imported echinococcosis have been increasing in Korea. The present study was undertaken to develop a serodiagnostic system for echinococcosis in Korea. For diagnosis of echinococcosis, the fluid of Echinococcus granulosus hydatid cysts was collected from naturally infected sheep in Uzbekistan. Also serum samples of infected patients who were surgically confirmed were collected in a hospital in Tashkent, Uzbekistan. According to the absorbance of 59 echinococcosis positive and 39 negative control serum samples, the cut-off value was determined as 0.27. The sensitivity and specificity of ELISA with hydatid fluid antigen were 91.5% and 96%, respectively. The antigen cross-reacted with the serum of some cysticercosis or clonorchiasis patients. However, immunoblot analysis on the cystic fluid recognized antigenic proteins of 7-, 16-, and 24-kDa bands in their dominant protein quantity and strong blotting reactivity. In conclusion, the present ELISA system using hydatid cyst fluid antigen from Uzbekistan sheep is sensitive and specific for diagnosis of echinococcosis cases. |
format | Online Article Text |
id | pubmed-3712105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Korean Society for Parasitology and Tropical Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-37121052013-07-17 Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep Jin, Yan Anvarov, Khikmat Khajibaev, Abdukhakim Hong, Samin Hong, Sung-Tae Korean J Parasitol Original Article According to increase of travel, the cases of imported echinococcosis have been increasing in Korea. The present study was undertaken to develop a serodiagnostic system for echinococcosis in Korea. For diagnosis of echinococcosis, the fluid of Echinococcus granulosus hydatid cysts was collected from naturally infected sheep in Uzbekistan. Also serum samples of infected patients who were surgically confirmed were collected in a hospital in Tashkent, Uzbekistan. According to the absorbance of 59 echinococcosis positive and 39 negative control serum samples, the cut-off value was determined as 0.27. The sensitivity and specificity of ELISA with hydatid fluid antigen were 91.5% and 96%, respectively. The antigen cross-reacted with the serum of some cysticercosis or clonorchiasis patients. However, immunoblot analysis on the cystic fluid recognized antigenic proteins of 7-, 16-, and 24-kDa bands in their dominant protein quantity and strong blotting reactivity. In conclusion, the present ELISA system using hydatid cyst fluid antigen from Uzbekistan sheep is sensitive and specific for diagnosis of echinococcosis cases. The Korean Society for Parasitology and Tropical Medicine 2013-06 2013-06-30 /pmc/articles/PMC3712105/ /pubmed/23864742 http://dx.doi.org/10.3347/kjp.2013.51.3.313 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jin, Yan Anvarov, Khikmat Khajibaev, Abdukhakim Hong, Samin Hong, Sung-Tae Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep |
title | Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep |
title_full | Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep |
title_fullStr | Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep |
title_full_unstemmed | Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep |
title_short | Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep |
title_sort | serodiagnosis of echinococcosis by elisa using cystic fluid from uzbekistan sheep |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712105/ https://www.ncbi.nlm.nih.gov/pubmed/23864742 http://dx.doi.org/10.3347/kjp.2013.51.3.313 |
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