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Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis
Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immuno...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society for Parasitology and Tropical Medicine
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712107/ https://www.ncbi.nlm.nih.gov/pubmed/23864744 http://dx.doi.org/10.3347/kjp.2013.51.3.327 |
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author | Hu, Yonghong Zhang, Jincheng Yang, Shujie Wang, Hui Zeng, Hua Zhang, Tiantian Liu, Jingze |
author_facet | Hu, Yonghong Zhang, Jincheng Yang, Shujie Wang, Hui Zeng, Hua Zhang, Tiantian Liu, Jingze |
author_sort | Hu, Yonghong |
collection | PubMed |
description | Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicated that it was a H. longicornis paramyosin (Hl-Pmy). The full lenghth cDNA of Hl-Pmy was obtained by rapid amplification of cDNA ends (RACE) which consisted of 2,783 bp with a 161 bp 3' untranslated region. Sequence alignment of tick paramyosin (Pmy) showed that Hl-Pmy shared a high level of conservation among ticks. Comparison with the protective epitope sequence of other invertebrate Pmy, it was calculated that the protective epitope of Hl-Pmy was a peptide (LEEAEGSSETVVEMNKKRDTE) named LEE, which was close to the N-terminal of Hl-Pmy protein. The secondary structure analysis suggested that LEE had non-helical segments within an α-helical structure. These results provide the basis for developing a vaccine against biting H. longicornis ticks. |
format | Online Article Text |
id | pubmed-3712107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Korean Society for Parasitology and Tropical Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-37121072013-07-17 Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis Hu, Yonghong Zhang, Jincheng Yang, Shujie Wang, Hui Zeng, Hua Zhang, Tiantian Liu, Jingze Korean J Parasitol Original Article Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicated that it was a H. longicornis paramyosin (Hl-Pmy). The full lenghth cDNA of Hl-Pmy was obtained by rapid amplification of cDNA ends (RACE) which consisted of 2,783 bp with a 161 bp 3' untranslated region. Sequence alignment of tick paramyosin (Pmy) showed that Hl-Pmy shared a high level of conservation among ticks. Comparison with the protective epitope sequence of other invertebrate Pmy, it was calculated that the protective epitope of Hl-Pmy was a peptide (LEEAEGSSETVVEMNKKRDTE) named LEE, which was close to the N-terminal of Hl-Pmy protein. The secondary structure analysis suggested that LEE had non-helical segments within an α-helical structure. These results provide the basis for developing a vaccine against biting H. longicornis ticks. The Korean Society for Parasitology and Tropical Medicine 2013-06 2013-06-30 /pmc/articles/PMC3712107/ /pubmed/23864744 http://dx.doi.org/10.3347/kjp.2013.51.3.327 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Hu, Yonghong Zhang, Jincheng Yang, Shujie Wang, Hui Zeng, Hua Zhang, Tiantian Liu, Jingze Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis |
title | Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis |
title_full | Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis |
title_fullStr | Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis |
title_full_unstemmed | Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis |
title_short | Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis |
title_sort | screening and molecular cloning of a protective antigen from the midgut of haemaphysalis longicornis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712107/ https://www.ncbi.nlm.nih.gov/pubmed/23864744 http://dx.doi.org/10.3347/kjp.2013.51.3.327 |
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