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Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes

Purpose. To evaluate the role of corneal epithelium in riboflavin/ultraviolet-A (UVA) mediated corneal collagen cross-linking treatment. Methods. Fifty New Zealand rabbits were divided into 5 groups: UVA treatment with or without corneal epithelium, UVA+riboflavin treatment with or without corneal e...

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Autores principales: Tao, Xiangchen, Yu, Haiqun, Zhang, Yong, Li, Zhiwei, Jhanji, Vishal, Ni, Shouxiang, Wang, Ya, Mu, Guoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712226/
https://www.ncbi.nlm.nih.gov/pubmed/23936826
http://dx.doi.org/10.1155/2013/624563
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author Tao, Xiangchen
Yu, Haiqun
Zhang, Yong
Li, Zhiwei
Jhanji, Vishal
Ni, Shouxiang
Wang, Ya
Mu, Guoying
author_facet Tao, Xiangchen
Yu, Haiqun
Zhang, Yong
Li, Zhiwei
Jhanji, Vishal
Ni, Shouxiang
Wang, Ya
Mu, Guoying
author_sort Tao, Xiangchen
collection PubMed
description Purpose. To evaluate the role of corneal epithelium in riboflavin/ultraviolet-A (UVA) mediated corneal collagen cross-linking treatment. Methods. Fifty New Zealand rabbits were divided into 5 groups: UVA treatment with or without corneal epithelium, UVA+riboflavin treatment with or without corneal epithelium, and control without any treatment. All rabbits were sacrificed after irradiation and subsequently 4 mm × 10 mm corneal strips were harvested for biomechanical evaluation. Results. UVA irradiation alone did not enhance the maximal stress and Young's modulus of corneal specimens with (3.15 ± 0.56 mpa, 1.00 ± 0.09 mpa) or without (3.53 ± 0.85 mpa, 0.94 ± 0.21 mpa) the corneal epithelium, compared to specimens in the control group (4.30 ± 0.68 mpa, 1.03 ± 0.24 mpa). However, UVA irradiation combined with riboflavin significantly increased the maximal stress and Young's modulus of corneal specimens with (5.27 ± 1.09 mpa, 1.23 ± 0.23 mpa, P < 0.05) or without (7.16 ± 1.88 mpa, 1.42 ± 0.16 mpa, P < 0.05) corneal epithelium when compared to the control group. The maximal stress and Young's modulus of cornea in UVA+riboflavin and “epithelium-off” group were 35.9% and 15.4% higher compared to the UVA+riboflavin and “epithelium-on” group, respectively (P < 0.05). Conclusions. Our study shows that UVA+riboflavin treatment significantly affects the biomechanical properties of the cornea with and without epithelial removal. However, corneas without epithelium seem to benefit more compared to corneas with the epithelium.
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spelling pubmed-37122262013-08-09 Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes Tao, Xiangchen Yu, Haiqun Zhang, Yong Li, Zhiwei Jhanji, Vishal Ni, Shouxiang Wang, Ya Mu, Guoying Biomed Res Int Research Article Purpose. To evaluate the role of corneal epithelium in riboflavin/ultraviolet-A (UVA) mediated corneal collagen cross-linking treatment. Methods. Fifty New Zealand rabbits were divided into 5 groups: UVA treatment with or without corneal epithelium, UVA+riboflavin treatment with or without corneal epithelium, and control without any treatment. All rabbits were sacrificed after irradiation and subsequently 4 mm × 10 mm corneal strips were harvested for biomechanical evaluation. Results. UVA irradiation alone did not enhance the maximal stress and Young's modulus of corneal specimens with (3.15 ± 0.56 mpa, 1.00 ± 0.09 mpa) or without (3.53 ± 0.85 mpa, 0.94 ± 0.21 mpa) the corneal epithelium, compared to specimens in the control group (4.30 ± 0.68 mpa, 1.03 ± 0.24 mpa). However, UVA irradiation combined with riboflavin significantly increased the maximal stress and Young's modulus of corneal specimens with (5.27 ± 1.09 mpa, 1.23 ± 0.23 mpa, P < 0.05) or without (7.16 ± 1.88 mpa, 1.42 ± 0.16 mpa, P < 0.05) corneal epithelium when compared to the control group. The maximal stress and Young's modulus of cornea in UVA+riboflavin and “epithelium-off” group were 35.9% and 15.4% higher compared to the UVA+riboflavin and “epithelium-on” group, respectively (P < 0.05). Conclusions. Our study shows that UVA+riboflavin treatment significantly affects the biomechanical properties of the cornea with and without epithelial removal. However, corneas without epithelium seem to benefit more compared to corneas with the epithelium. Hindawi Publishing Corporation 2013 2013-06-27 /pmc/articles/PMC3712226/ /pubmed/23936826 http://dx.doi.org/10.1155/2013/624563 Text en Copyright © 2013 Xiangchen Tao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tao, Xiangchen
Yu, Haiqun
Zhang, Yong
Li, Zhiwei
Jhanji, Vishal
Ni, Shouxiang
Wang, Ya
Mu, Guoying
Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes
title Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes
title_full Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes
title_fullStr Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes
title_full_unstemmed Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes
title_short Role of Corneal Epithelium in Riboflavin/Ultraviolet-A Mediated Corneal Cross-linking Treatment in Rabbit Eyes
title_sort role of corneal epithelium in riboflavin/ultraviolet-a mediated corneal cross-linking treatment in rabbit eyes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712226/
https://www.ncbi.nlm.nih.gov/pubmed/23936826
http://dx.doi.org/10.1155/2013/624563
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