Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed...

Descripción completa

Detalles Bibliográficos
Autores principales: Ke, Rongqin, Nong, Rachel Yuan, Fredriksson, Simon, Landegren, Ulf, Nilsson, Mats
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713053/
https://www.ncbi.nlm.nih.gov/pubmed/23874999
http://dx.doi.org/10.1371/journal.pone.0069813
_version_ 1782277151776571392
author Ke, Rongqin
Nong, Rachel Yuan
Fredriksson, Simon
Landegren, Ulf
Nilsson, Mats
author_facet Ke, Rongqin
Nong, Rachel Yuan
Fredriksson, Simon
Landegren, Ulf
Nilsson, Mats
author_sort Ke, Rongqin
collection PubMed
description Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout.
format Online
Article
Text
id pubmed-3713053
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37130532013-07-19 Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection Ke, Rongqin Nong, Rachel Yuan Fredriksson, Simon Landegren, Ulf Nilsson, Mats PLoS One Research Article Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout. Public Library of Science 2013-07-16 /pmc/articles/PMC3713053/ /pubmed/23874999 http://dx.doi.org/10.1371/journal.pone.0069813 Text en © 2013 Ke et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ke, Rongqin
Nong, Rachel Yuan
Fredriksson, Simon
Landegren, Ulf
Nilsson, Mats
Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
title Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
title_full Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
title_fullStr Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
title_full_unstemmed Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
title_short Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
title_sort improving precision of proximity ligation assay by amplified single molecule detection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713053/
https://www.ncbi.nlm.nih.gov/pubmed/23874999
http://dx.doi.org/10.1371/journal.pone.0069813
work_keys_str_mv AT kerongqin improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT nongrachelyuan improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT fredrikssonsimon improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT landegrenulf improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection
AT nilssonmats improvingprecisionofproximityligationassaybyamplifiedsinglemoleculedetection

Ejemplares similares