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microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report

Uterine sarcomas and mixed epithelial–mesenchymal uterine tumors are a heterogeneous group of rare tumors for which there are very few diagnostic markers available. As aberrant microRNA (miRNA) expression patterns represent putative diagnostic cancer markers, we aimed to identify miRNA expression pr...

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Autores principales: Kowalewska, Magdalena, Bakula-Zalewska, Elwira, Chechlinska, Magdalena, Goryca, Krzysztof, Nasierowska-Guttmejer, Anna, Danska-Bidzinska, Anna, Bidzinski, Mariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713270/
https://www.ncbi.nlm.nih.gov/pubmed/23558962
http://dx.doi.org/10.1007/s13277-013-0748-5
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author Kowalewska, Magdalena
Bakula-Zalewska, Elwira
Chechlinska, Magdalena
Goryca, Krzysztof
Nasierowska-Guttmejer, Anna
Danska-Bidzinska, Anna
Bidzinski, Mariusz
author_facet Kowalewska, Magdalena
Bakula-Zalewska, Elwira
Chechlinska, Magdalena
Goryca, Krzysztof
Nasierowska-Guttmejer, Anna
Danska-Bidzinska, Anna
Bidzinski, Mariusz
author_sort Kowalewska, Magdalena
collection PubMed
description Uterine sarcomas and mixed epithelial–mesenchymal uterine tumors are a heterogeneous group of rare tumors for which there are very few diagnostic markers available. As aberrant microRNA (miRNA) expression patterns represent putative diagnostic cancer markers, we aimed to identify miRNA expression profiles of the major uterine sarcoma subtypes and mixed epithelial–mesenchymal tumors of the uterus. Eighty-eight miRNAs were assessed by quantitative RT-PCR in cancerous and non-cancerous tissue samples collected from 29 patients with endometrial sarcoma, leiomyosarcoma, and mixed epithelial–mesenchymal tumors. Tumor and control samples significantly (P < 0.05) differed in the expression of miR-23b, miR-1, let-7f, and let-7c in endometrial sarcomas, and miR-1, let-7c, miR-133b, let-7b, miR-143, let-7a, let-7d, let-7e, let-7g, miR-222, let-7i, and miR-214 in mixed epithelial–mesenchymal tumors. All the significantly changed miRNAs were down-regulated in the malignant tissues as compared to their normal counterparts. This may suggest their tumor suppressor role in these malignancies. No statistically significant changes in miRNA expression levels were found between leiomyosarcoma tumors and controls. The identified miRNAs warrant further studies as valuable candidate markers for the differential diagnosis of uterine sarcomas from benign uterine lesions and between uterine sarcoma subtypes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13277-013-0748-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-37132702013-08-15 microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report Kowalewska, Magdalena Bakula-Zalewska, Elwira Chechlinska, Magdalena Goryca, Krzysztof Nasierowska-Guttmejer, Anna Danska-Bidzinska, Anna Bidzinski, Mariusz Tumour Biol Research Article Uterine sarcomas and mixed epithelial–mesenchymal uterine tumors are a heterogeneous group of rare tumors for which there are very few diagnostic markers available. As aberrant microRNA (miRNA) expression patterns represent putative diagnostic cancer markers, we aimed to identify miRNA expression profiles of the major uterine sarcoma subtypes and mixed epithelial–mesenchymal tumors of the uterus. Eighty-eight miRNAs were assessed by quantitative RT-PCR in cancerous and non-cancerous tissue samples collected from 29 patients with endometrial sarcoma, leiomyosarcoma, and mixed epithelial–mesenchymal tumors. Tumor and control samples significantly (P < 0.05) differed in the expression of miR-23b, miR-1, let-7f, and let-7c in endometrial sarcomas, and miR-1, let-7c, miR-133b, let-7b, miR-143, let-7a, let-7d, let-7e, let-7g, miR-222, let-7i, and miR-214 in mixed epithelial–mesenchymal tumors. All the significantly changed miRNAs were down-regulated in the malignant tissues as compared to their normal counterparts. This may suggest their tumor suppressor role in these malignancies. No statistically significant changes in miRNA expression levels were found between leiomyosarcoma tumors and controls. The identified miRNAs warrant further studies as valuable candidate markers for the differential diagnosis of uterine sarcomas from benign uterine lesions and between uterine sarcoma subtypes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13277-013-0748-5) contains supplementary material, which is available to authorized users. Springer Netherlands 2013-04-05 /pmc/articles/PMC3713270/ /pubmed/23558962 http://dx.doi.org/10.1007/s13277-013-0748-5 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research Article
Kowalewska, Magdalena
Bakula-Zalewska, Elwira
Chechlinska, Magdalena
Goryca, Krzysztof
Nasierowska-Guttmejer, Anna
Danska-Bidzinska, Anna
Bidzinski, Mariusz
microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report
title microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report
title_full microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report
title_fullStr microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report
title_full_unstemmed microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report
title_short microRNAs in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report
title_sort micrornas in uterine sarcomas and mixed epithelial–mesenchymal uterine tumors: a preliminary report
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713270/
https://www.ncbi.nlm.nih.gov/pubmed/23558962
http://dx.doi.org/10.1007/s13277-013-0748-5
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