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The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
AIMS: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. MATERIALS AND METHODS: Mature male wistar rats (n...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713579/ https://www.ncbi.nlm.nih.gov/pubmed/23869153 http://dx.doi.org/10.4103/0974-1208.112383 |
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author | Hemadi, Masoud Saki, Ghasem Rajabzadeh, Asghar Khodadadi, Ali Sarkaki, Alireza |
author_facet | Hemadi, Masoud Saki, Ghasem Rajabzadeh, Asghar Khodadadi, Ali Sarkaki, Alireza |
author_sort | Hemadi, Masoud |
collection | PubMed |
description | AIMS: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. MATERIALS AND METHODS: Mature male wistar rats (n = 24) were randomly grouped as follows: Group 1 (honey + noise stress), 2 (vitamin E + noise stress), 3 (noise stress,) and 4 as the control group. In groups 1, 2, and 3, rats were exposed to noise stress. In groups 1 and 2, rats also were given honey and vitamin E, respectively, orally for 50 days. After that, the germinative and somatic cells of testes parenchyma were isolated by digesting the whole testes by a standard method. Next, viability, apoptosis, and necrosis of the cells were evaluated by TUNEL kit and flow cytometry. RESULTS: The rates of apoptosis and necrosis of the testicular cells were increased (P = 0.003 and P = 0.001, respectively), but viability of these cells decreased in testes of rats exposed to noise stress (P = 0.003). However, administration of honey and vitamin E were significantly helpful in keeping the cells of testis parenchyma alive, which suffers from noise pollution (P < 0.05 and P < 0.05, respectively). CONCLUSIONS: Noise stress has negative influences on the cells of testicular tissue by increasing apoptotic and necrotic cells. However, the associated enhancement in healthy cells suggests that honey and vitamin E have positive influences on the testis parenchyma. |
format | Online Article Text |
id | pubmed-3713579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37135792013-07-18 The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress Hemadi, Masoud Saki, Ghasem Rajabzadeh, Asghar Khodadadi, Ali Sarkaki, Alireza J Hum Reprod Sci Original Article AIMS: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. MATERIALS AND METHODS: Mature male wistar rats (n = 24) were randomly grouped as follows: Group 1 (honey + noise stress), 2 (vitamin E + noise stress), 3 (noise stress,) and 4 as the control group. In groups 1, 2, and 3, rats were exposed to noise stress. In groups 1 and 2, rats also were given honey and vitamin E, respectively, orally for 50 days. After that, the germinative and somatic cells of testes parenchyma were isolated by digesting the whole testes by a standard method. Next, viability, apoptosis, and necrosis of the cells were evaluated by TUNEL kit and flow cytometry. RESULTS: The rates of apoptosis and necrosis of the testicular cells were increased (P = 0.003 and P = 0.001, respectively), but viability of these cells decreased in testes of rats exposed to noise stress (P = 0.003). However, administration of honey and vitamin E were significantly helpful in keeping the cells of testis parenchyma alive, which suffers from noise pollution (P < 0.05 and P < 0.05, respectively). CONCLUSIONS: Noise stress has negative influences on the cells of testicular tissue by increasing apoptotic and necrotic cells. However, the associated enhancement in healthy cells suggests that honey and vitamin E have positive influences on the testis parenchyma. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3713579/ /pubmed/23869153 http://dx.doi.org/10.4103/0974-1208.112383 Text en Copyright: © Journal of Human Reproductive Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Hemadi, Masoud Saki, Ghasem Rajabzadeh, Asghar Khodadadi, Ali Sarkaki, Alireza The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress |
title | The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress |
title_full | The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress |
title_fullStr | The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress |
title_full_unstemmed | The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress |
title_short | The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress |
title_sort | effects of honey and vitamin e administration on apoptosis in testes of rat exposed to noise stress |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713579/ https://www.ncbi.nlm.nih.gov/pubmed/23869153 http://dx.doi.org/10.4103/0974-1208.112383 |
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