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The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress

AIMS: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. MATERIALS AND METHODS: Mature male wistar rats (n...

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Autores principales: Hemadi, Masoud, Saki, Ghasem, Rajabzadeh, Asghar, Khodadadi, Ali, Sarkaki, Alireza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713579/
https://www.ncbi.nlm.nih.gov/pubmed/23869153
http://dx.doi.org/10.4103/0974-1208.112383
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author Hemadi, Masoud
Saki, Ghasem
Rajabzadeh, Asghar
Khodadadi, Ali
Sarkaki, Alireza
author_facet Hemadi, Masoud
Saki, Ghasem
Rajabzadeh, Asghar
Khodadadi, Ali
Sarkaki, Alireza
author_sort Hemadi, Masoud
collection PubMed
description AIMS: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. MATERIALS AND METHODS: Mature male wistar rats (n = 24) were randomly grouped as follows: Group 1 (honey + noise stress), 2 (vitamin E + noise stress), 3 (noise stress,) and 4 as the control group. In groups 1, 2, and 3, rats were exposed to noise stress. In groups 1 and 2, rats also were given honey and vitamin E, respectively, orally for 50 days. After that, the germinative and somatic cells of testes parenchyma were isolated by digesting the whole testes by a standard method. Next, viability, apoptosis, and necrosis of the cells were evaluated by TUNEL kit and flow cytometry. RESULTS: The rates of apoptosis and necrosis of the testicular cells were increased (P = 0.003 and P = 0.001, respectively), but viability of these cells decreased in testes of rats exposed to noise stress (P = 0.003). However, administration of honey and vitamin E were significantly helpful in keeping the cells of testis parenchyma alive, which suffers from noise pollution (P < 0.05 and P < 0.05, respectively). CONCLUSIONS: Noise stress has negative influences on the cells of testicular tissue by increasing apoptotic and necrotic cells. However, the associated enhancement in healthy cells suggests that honey and vitamin E have positive influences on the testis parenchyma.
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spelling pubmed-37135792013-07-18 The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress Hemadi, Masoud Saki, Ghasem Rajabzadeh, Asghar Khodadadi, Ali Sarkaki, Alireza J Hum Reprod Sci Original Article AIMS: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. MATERIALS AND METHODS: Mature male wistar rats (n = 24) were randomly grouped as follows: Group 1 (honey + noise stress), 2 (vitamin E + noise stress), 3 (noise stress,) and 4 as the control group. In groups 1, 2, and 3, rats were exposed to noise stress. In groups 1 and 2, rats also were given honey and vitamin E, respectively, orally for 50 days. After that, the germinative and somatic cells of testes parenchyma were isolated by digesting the whole testes by a standard method. Next, viability, apoptosis, and necrosis of the cells were evaluated by TUNEL kit and flow cytometry. RESULTS: The rates of apoptosis and necrosis of the testicular cells were increased (P = 0.003 and P = 0.001, respectively), but viability of these cells decreased in testes of rats exposed to noise stress (P = 0.003). However, administration of honey and vitamin E were significantly helpful in keeping the cells of testis parenchyma alive, which suffers from noise pollution (P < 0.05 and P < 0.05, respectively). CONCLUSIONS: Noise stress has negative influences on the cells of testicular tissue by increasing apoptotic and necrotic cells. However, the associated enhancement in healthy cells suggests that honey and vitamin E have positive influences on the testis parenchyma. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3713579/ /pubmed/23869153 http://dx.doi.org/10.4103/0974-1208.112383 Text en Copyright: © Journal of Human Reproductive Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hemadi, Masoud
Saki, Ghasem
Rajabzadeh, Asghar
Khodadadi, Ali
Sarkaki, Alireza
The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
title The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
title_full The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
title_fullStr The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
title_full_unstemmed The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
title_short The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress
title_sort effects of honey and vitamin e administration on apoptosis in testes of rat exposed to noise stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3713579/
https://www.ncbi.nlm.nih.gov/pubmed/23869153
http://dx.doi.org/10.4103/0974-1208.112383
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