Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein

CDK5RAP2 is a centrosomal protein known to be involved in the regulation of the γ-tubulin ring complex and thus the organization of microtubule arrays. However, the mechanism by which CDK5RAP2 is itself recruited to centrosomes is poorly understood. We report here that CDK5RAP2 displays highly dynam...

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Detalles Bibliográficos
Autores principales: Jia, Yue, Fong, Ka-Wing, Choi, Yuk-Kwan, See, Siu-San, Qi, Robert Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714271/
https://www.ncbi.nlm.nih.gov/pubmed/23874654
http://dx.doi.org/10.1371/journal.pone.0068523
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author Jia, Yue
Fong, Ka-Wing
Choi, Yuk-Kwan
See, Siu-San
Qi, Robert Z.
author_facet Jia, Yue
Fong, Ka-Wing
Choi, Yuk-Kwan
See, Siu-San
Qi, Robert Z.
author_sort Jia, Yue
collection PubMed
description CDK5RAP2 is a centrosomal protein known to be involved in the regulation of the γ-tubulin ring complex and thus the organization of microtubule arrays. However, the mechanism by which CDK5RAP2 is itself recruited to centrosomes is poorly understood. We report here that CDK5RAP2 displays highly dynamic attachment to centrosomes in a microtubule-dependent manner. CDK5RAP2 associates with the retrograde transporter dynein-dynactin and contains a sequence motif that binds to dynein light chain 8. Significantly, disruption of cellular dynein-dynactin function reduces the centrosomal level of CDK5RAP2. These results reveal a key role of the dynein-dynactin complex in the dynamic recruitment of CDK5RAP2 to centrosomes.
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spelling pubmed-37142712013-07-19 Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein Jia, Yue Fong, Ka-Wing Choi, Yuk-Kwan See, Siu-San Qi, Robert Z. PLoS One Research Article CDK5RAP2 is a centrosomal protein known to be involved in the regulation of the γ-tubulin ring complex and thus the organization of microtubule arrays. However, the mechanism by which CDK5RAP2 is itself recruited to centrosomes is poorly understood. We report here that CDK5RAP2 displays highly dynamic attachment to centrosomes in a microtubule-dependent manner. CDK5RAP2 associates with the retrograde transporter dynein-dynactin and contains a sequence motif that binds to dynein light chain 8. Significantly, disruption of cellular dynein-dynactin function reduces the centrosomal level of CDK5RAP2. These results reveal a key role of the dynein-dynactin complex in the dynamic recruitment of CDK5RAP2 to centrosomes. Public Library of Science 2013-07-17 /pmc/articles/PMC3714271/ /pubmed/23874654 http://dx.doi.org/10.1371/journal.pone.0068523 Text en © 2013 Jia et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jia, Yue
Fong, Ka-Wing
Choi, Yuk-Kwan
See, Siu-San
Qi, Robert Z.
Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein
title Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein
title_full Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein
title_fullStr Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein
title_full_unstemmed Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein
title_short Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein
title_sort dynamic recruitment of cdk5rap2 to centrosomes requires its association with dynein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714271/
https://www.ncbi.nlm.nih.gov/pubmed/23874654
http://dx.doi.org/10.1371/journal.pone.0068523
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