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Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus

BACKGROUND/AIMS: Detailed characterization of the ultrastructural morphology of intercellular space in gastroesophageal reflux disease has not been fully studied. We aimed to investigate whether subtle alteration in intercellular space structure and tight junction proteins might differ among patient...

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Autores principales: Liu, Chia-Chin, Lee, Jeng Woei, Liu, Tso-Tsai, Yi, Chih-Hsun, Chen, Chien-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Neurogastroenterology and Motility 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714410/
https://www.ncbi.nlm.nih.gov/pubmed/23875099
http://dx.doi.org/10.5056/jnm.2013.19.3.324
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author Liu, Chia-Chin
Lee, Jeng Woei
Liu, Tso-Tsai
Yi, Chih-Hsun
Chen, Chien-Lin
author_facet Liu, Chia-Chin
Lee, Jeng Woei
Liu, Tso-Tsai
Yi, Chih-Hsun
Chen, Chien-Lin
author_sort Liu, Chia-Chin
collection PubMed
description BACKGROUND/AIMS: Detailed characterization of the ultrastructural morphology of intercellular space in gastroesophageal reflux disease has not been fully studied. We aimed to investigate whether subtle alteration in intercellular space structure and tight junction proteins might differ among patients with gastroesophageal reflux disease. METHODS: Esophageal biopsies at 5 cm above the gastroesophageal junction were obtained from 6 asymptomatic controls, 10 patients with reflux symptoms but without erosions, and 18 patients with erosions. The biopsies were morphologically evaluated by transmission electron microscopy, and by using immunohistochemistry for tight junction proteins (claudin-1 and claudin-2 proteins). RESULTS: The expressions of tight junction proteins did not differ between asymptomatic controls and gastroesophageal reflux disease patients. In patients with gastroesophageal reflux disease, altered desmosomal junction morphology was only found in upper stratified squamous epithelium. Dilated intercellular space occurred only in upper stratified squamous epithelium and in patients with erosive esophagitis. CONCLUSIONS: This study suggests that dilated intercellular space may not be uniformly present inside the esophageal mucosa and predominantly it is located in upper squamous epithelium. Presence of desmosomal junction alterations is associated with increased severity of gastroesophageal reflux disease. Besides dilated intercellular space, subtle changes in ultrastructural morphology of intercellular space allow better identification of inflamed esophageal mucosa relevant to acid reflux.
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spelling pubmed-37144102013-07-19 Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus Liu, Chia-Chin Lee, Jeng Woei Liu, Tso-Tsai Yi, Chih-Hsun Chen, Chien-Lin J Neurogastroenterol Motil Original Article BACKGROUND/AIMS: Detailed characterization of the ultrastructural morphology of intercellular space in gastroesophageal reflux disease has not been fully studied. We aimed to investigate whether subtle alteration in intercellular space structure and tight junction proteins might differ among patients with gastroesophageal reflux disease. METHODS: Esophageal biopsies at 5 cm above the gastroesophageal junction were obtained from 6 asymptomatic controls, 10 patients with reflux symptoms but without erosions, and 18 patients with erosions. The biopsies were morphologically evaluated by transmission electron microscopy, and by using immunohistochemistry for tight junction proteins (claudin-1 and claudin-2 proteins). RESULTS: The expressions of tight junction proteins did not differ between asymptomatic controls and gastroesophageal reflux disease patients. In patients with gastroesophageal reflux disease, altered desmosomal junction morphology was only found in upper stratified squamous epithelium. Dilated intercellular space occurred only in upper stratified squamous epithelium and in patients with erosive esophagitis. CONCLUSIONS: This study suggests that dilated intercellular space may not be uniformly present inside the esophageal mucosa and predominantly it is located in upper squamous epithelium. Presence of desmosomal junction alterations is associated with increased severity of gastroesophageal reflux disease. Besides dilated intercellular space, subtle changes in ultrastructural morphology of intercellular space allow better identification of inflamed esophageal mucosa relevant to acid reflux. Korean Society of Neurogastroenterology and Motility 2013-07 2013-07-08 /pmc/articles/PMC3714410/ /pubmed/23875099 http://dx.doi.org/10.5056/jnm.2013.19.3.324 Text en © 2013 The Korean Society of Neurogastroenterology and Motility http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Liu, Chia-Chin
Lee, Jeng Woei
Liu, Tso-Tsai
Yi, Chih-Hsun
Chen, Chien-Lin
Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus
title Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus
title_full Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus
title_fullStr Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus
title_full_unstemmed Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus
title_short Relevance of Ultrastructural Alterations of Intercellular Junction Morphology in Inflamed Human Esophagus
title_sort relevance of ultrastructural alterations of intercellular junction morphology in inflamed human esophagus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714410/
https://www.ncbi.nlm.nih.gov/pubmed/23875099
http://dx.doi.org/10.5056/jnm.2013.19.3.324
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