Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System

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Here, we present a simple and highly efficient method for generating and detecting mutations of any gene in Drosophila melanogaster through the use of the CRISPR/Cas9 system (clustered regularly interspaced palindromic repeats/CRISPR-associated). We show that injection of RNA into the Drosophila emb...

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Detalles Bibliográficos
Autores principales: Bassett, Andrew R., Tibbit, Charlotte, Ponting, Chris P., Liu, Ji-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2013
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714591/
https://www.ncbi.nlm.nih.gov/pubmed/23827738
http://dx.doi.org/10.1016/j.celrep.2013.06.020

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