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DNA Extraction Systematics for Spectroscopic Studies

Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows p...

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Detalles Bibliográficos
Autores principales: Palma, Bianca Fogazza, Ferrari, Amanda Borges, Bitar, Renata Andrade, Cardoso, Maria Angélica Gargione, Martin, Airton A., da Silva Martinho, Herculano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3714657/
https://www.ncbi.nlm.nih.gov/pubmed/27879896
http://dx.doi.org/10.3390/s8063624
Descripción
Sumario:Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows probe molecular vibrations of genetic material in a fast way. The present work established a systematic way for extract DNA in suitable concentrations and structural integrity allowing studies by Raman spectroscopy or other spectroscopic technique, including bio-analytical sensors for probing genetic alterations.