Cargando…

Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells

Apoptosis or necrosis of neurons in the central nervous system (CNS) is the hallmark of many neurodegenerative diseases and Traumatic Brain Injury (TBI). The inability to regenerate in CNS offers little hope for naturally repairing the damaged neurons. However, with the rapid development of new tech...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Hong, Gupta, Anju, Zou, Tie, Zhou, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3715808/
_version_ 1782277506521366528
author Zhou, Hong
Gupta, Anju
Zou, Tie
Zhou, Jianhua
author_facet Zhou, Hong
Gupta, Anju
Zou, Tie
Zhou, Jianhua
author_sort Zhou, Hong
collection PubMed
description Apoptosis or necrosis of neurons in the central nervous system (CNS) is the hallmark of many neurodegenerative diseases and Traumatic Brain Injury (TBI). The inability to regenerate in CNS offers little hope for naturally repairing the damaged neurons. However, with the rapid development of new technologies, regenerative medicine offers great promises to patients with these disorders. Among many events for further advancement of regenerative medicine, extracellular matrix (ECM) plays a critical role for cellular migration and differentiation. To develop a biocompatible and electrically conductive substrate that can be potentially used to promote growth and regeneration of neurons and to record intracellular and multisite signals from brain as a probe, a polymeric precursor – SPR 220.7 was fabricated by pyrolysis at temperatures higher than 700 ºC. Human Neuroblastoma cells - SK-N-MC, SY5Y, mouse teratocarcinoma cells P-19 and rat PC12 cells were found to attach and proliferate on photoresist derived carbon film. Significantly, neuronal differentiation of PC12 cells induced by NGF was demonstrated by observing cell shape and size, and measuring the length of neurites under SEM. Our results indicated that fabricated carbon could potentially be explored in regenerative medicine for promoting neuronal growth and differentiation in CNS with neurodegeneration.
format Online
Article
Text
id pubmed-3715808
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-37158082013-07-19 Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells Zhou, Hong Gupta, Anju Zou, Tie Zhou, Jianhua Int J Mol Sci Full Research Paper Apoptosis or necrosis of neurons in the central nervous system (CNS) is the hallmark of many neurodegenerative diseases and Traumatic Brain Injury (TBI). The inability to regenerate in CNS offers little hope for naturally repairing the damaged neurons. However, with the rapid development of new technologies, regenerative medicine offers great promises to patients with these disorders. Among many events for further advancement of regenerative medicine, extracellular matrix (ECM) plays a critical role for cellular migration and differentiation. To develop a biocompatible and electrically conductive substrate that can be potentially used to promote growth and regeneration of neurons and to record intracellular and multisite signals from brain as a probe, a polymeric precursor – SPR 220.7 was fabricated by pyrolysis at temperatures higher than 700 ºC. Human Neuroblastoma cells - SK-N-MC, SY5Y, mouse teratocarcinoma cells P-19 and rat PC12 cells were found to attach and proliferate on photoresist derived carbon film. Significantly, neuronal differentiation of PC12 cells induced by NGF was demonstrated by observing cell shape and size, and measuring the length of neurites under SEM. Our results indicated that fabricated carbon could potentially be explored in regenerative medicine for promoting neuronal growth and differentiation in CNS with neurodegeneration. Molecular Diversity Preservation International (MDPI) 2007-08-27 /pmc/articles/PMC3715808/ Text en © 2007 by MDPI Reproduction is permitted for noncommercial purposes.
spellingShingle Full Research Paper
Zhou, Hong
Gupta, Anju
Zou, Tie
Zhou, Jianhua
Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells
title Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells
title_full Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells
title_fullStr Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells
title_full_unstemmed Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells
title_short Photoresist Derived Carbon for Growth and Differentiation of Neuronal Cells
title_sort photoresist derived carbon for growth and differentiation of neuronal cells
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3715808/
work_keys_str_mv AT zhouhong photoresistderivedcarbonforgrowthanddifferentiationofneuronalcells
AT guptaanju photoresistderivedcarbonforgrowthanddifferentiationofneuronalcells
AT zoutie photoresistderivedcarbonforgrowthanddifferentiationofneuronalcells
AT zhoujianhua photoresistderivedcarbonforgrowthanddifferentiationofneuronalcells