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Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don

BACKGROUND: Angiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the anti...

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Autores principales: Dai, Zhi-Jun, Lu, Wang-Feng, Gao, Jie, Kang, Hua-Feng, Ma, Yu-Guang, Zhang, Shu-Qun, Diao, Yan, Lin, Shuai, Wang, Xi-Jing, Wu, Wen-Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3717098/
https://www.ncbi.nlm.nih.gov/pubmed/23815868
http://dx.doi.org/10.1186/1472-6882-13-150
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author Dai, Zhi-Jun
Lu, Wang-Feng
Gao, Jie
Kang, Hua-Feng
Ma, Yu-Guang
Zhang, Shu-Qun
Diao, Yan
Lin, Shuai
Wang, Xi-Jing
Wu, Wen-Ying
author_facet Dai, Zhi-Jun
Lu, Wang-Feng
Gao, Jie
Kang, Hua-Feng
Ma, Yu-Guang
Zhang, Shu-Qun
Diao, Yan
Lin, Shuai
Wang, Xi-Jing
Wu, Wen-Ying
author_sort Dai, Zhi-Jun
collection PubMed
description BACKGROUND: Angiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the antitumor molecular mechanism of S. barbata was still unclear. This study aimed to investigate the inhibitory effects of the total flavones in S. barbata (TF-SB) on angiogenesis. METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with various concentrations of TF-SB. Cell viability was examined using the MTT assay. The scratch assay was used to detect the migration of HUVECs after treatment with TF-SB. The ability of HUVECs to form network structures in vitro was demonstrated using the tube formation assay. The chick embryo chorioallantoic membrane assay was performed to detect the in vivo anti-angiogenic effect. The expression of VEGF was measured by the enzyme-linked immunosorbent. RESULTS: Results showed that TF-SB inhibited the proliferation and migration of HUVECs in a dose- dependent manner. Simultaneously, TF-SB significantly suppressed HUVEC angiogenesis in vitro and in vivo. Furthermore, VEGF was downregulated in both HUVECs and MHCC97-H cells after TF-SB treatment. CONCLUSION: TF-SB could suppress the process of angiogenesis in vitro and in vivo. TF-SB potentially suppresses angiogenesis in HUVECs by regulating VEGF. These findings suggested that TF-SB may serve as a potent anti-angiogenic agent.
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spelling pubmed-37170982013-07-21 Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don Dai, Zhi-Jun Lu, Wang-Feng Gao, Jie Kang, Hua-Feng Ma, Yu-Guang Zhang, Shu-Qun Diao, Yan Lin, Shuai Wang, Xi-Jing Wu, Wen-Ying BMC Complement Altern Med Research Article BACKGROUND: Angiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the antitumor molecular mechanism of S. barbata was still unclear. This study aimed to investigate the inhibitory effects of the total flavones in S. barbata (TF-SB) on angiogenesis. METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with various concentrations of TF-SB. Cell viability was examined using the MTT assay. The scratch assay was used to detect the migration of HUVECs after treatment with TF-SB. The ability of HUVECs to form network structures in vitro was demonstrated using the tube formation assay. The chick embryo chorioallantoic membrane assay was performed to detect the in vivo anti-angiogenic effect. The expression of VEGF was measured by the enzyme-linked immunosorbent. RESULTS: Results showed that TF-SB inhibited the proliferation and migration of HUVECs in a dose- dependent manner. Simultaneously, TF-SB significantly suppressed HUVEC angiogenesis in vitro and in vivo. Furthermore, VEGF was downregulated in both HUVECs and MHCC97-H cells after TF-SB treatment. CONCLUSION: TF-SB could suppress the process of angiogenesis in vitro and in vivo. TF-SB potentially suppresses angiogenesis in HUVECs by regulating VEGF. These findings suggested that TF-SB may serve as a potent anti-angiogenic agent. BioMed Central 2013-07-01 /pmc/articles/PMC3717098/ /pubmed/23815868 http://dx.doi.org/10.1186/1472-6882-13-150 Text en Copyright © 2013 Dai et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dai, Zhi-Jun
Lu, Wang-Feng
Gao, Jie
Kang, Hua-Feng
Ma, Yu-Guang
Zhang, Shu-Qun
Diao, Yan
Lin, Shuai
Wang, Xi-Jing
Wu, Wen-Ying
Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don
title Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don
title_full Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don
title_fullStr Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don
title_full_unstemmed Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don
title_short Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don
title_sort anti-angiogenic effect of the total flavonoids in scutellaria barbata d. don
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3717098/
https://www.ncbi.nlm.nih.gov/pubmed/23815868
http://dx.doi.org/10.1186/1472-6882-13-150
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