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Nutrition and degeneration of articular cartilage

PURPOSE: To determine the importance of synovial fluid (SF) or subchondral bone marrow (BM) as nutrition sources in cartilage degeneration. METHODS: Ninety-five-month-old male rabbits were randomly divided into 5 groups according to sources of nutrition: SFBM-both; BM-only; SF-only; None-SFBM; and F...

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Autores principales: Wang, Yuze, Wei, Lei, Zeng, Lingyuan, He, Dongdong, Wei, Xiaochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3717153/
https://www.ncbi.nlm.nih.gov/pubmed/22476522
http://dx.doi.org/10.1007/s00167-012-1977-7
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author Wang, Yuze
Wei, Lei
Zeng, Lingyuan
He, Dongdong
Wei, Xiaochun
author_facet Wang, Yuze
Wei, Lei
Zeng, Lingyuan
He, Dongdong
Wei, Xiaochun
author_sort Wang, Yuze
collection PubMed
description PURPOSE: To determine the importance of synovial fluid (SF) or subchondral bone marrow (BM) as nutrition sources in cartilage degeneration. METHODS: Ninety-five-month-old male rabbits were randomly divided into 5 groups according to sources of nutrition: SFBM-both; BM-only; SF-only; None-SFBM; and Free plug (unrestricted). Nutrition to 4-mm-diameter cylindrical osteochondral plugs created on the trochlea of the distal femurs was obstructed by Polyvinyl Chloride (PVC) cap. Cartilage changes were assessed after 4, 8, and 12 weeks by histology, immunohistochemistry, and real-time PCR. RESULTS: Cartilage in the BM-only group suffered the greatest damage, followed by the None-SFBM and SF-only groups. Apoptosis was increased in the BM-only and None-SFBM groups compared with others. Cartilage was significantly thinner at all time points in the BM-only and None-SFBM groups when compared with SFBM-both and Free plug, whereas in the SF-only group, this difference occurred after 8 weeks. Compared with SFBM-both and Free plug, expression of collagen II and aggrecan mRNAs in all groups was decreased but MMP-3 increased, respectively. CONCLUSION: Our data indicate that SF-derived nutrition is the dominant source of sustenance for adult cartilage structure and function. Cartilage damage is observed when the only nutrition source is the BM. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00167-012-1977-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-37171532013-07-23 Nutrition and degeneration of articular cartilage Wang, Yuze Wei, Lei Zeng, Lingyuan He, Dongdong Wei, Xiaochun Knee Surg Sports Traumatol Arthrosc Experimental Study PURPOSE: To determine the importance of synovial fluid (SF) or subchondral bone marrow (BM) as nutrition sources in cartilage degeneration. METHODS: Ninety-five-month-old male rabbits were randomly divided into 5 groups according to sources of nutrition: SFBM-both; BM-only; SF-only; None-SFBM; and Free plug (unrestricted). Nutrition to 4-mm-diameter cylindrical osteochondral plugs created on the trochlea of the distal femurs was obstructed by Polyvinyl Chloride (PVC) cap. Cartilage changes were assessed after 4, 8, and 12 weeks by histology, immunohistochemistry, and real-time PCR. RESULTS: Cartilage in the BM-only group suffered the greatest damage, followed by the None-SFBM and SF-only groups. Apoptosis was increased in the BM-only and None-SFBM groups compared with others. Cartilage was significantly thinner at all time points in the BM-only and None-SFBM groups when compared with SFBM-both and Free plug, whereas in the SF-only group, this difference occurred after 8 weeks. Compared with SFBM-both and Free plug, expression of collagen II and aggrecan mRNAs in all groups was decreased but MMP-3 increased, respectively. CONCLUSION: Our data indicate that SF-derived nutrition is the dominant source of sustenance for adult cartilage structure and function. Cartilage damage is observed when the only nutrition source is the BM. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00167-012-1977-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2012-04-04 2013 /pmc/articles/PMC3717153/ /pubmed/22476522 http://dx.doi.org/10.1007/s00167-012-1977-7 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Experimental Study
Wang, Yuze
Wei, Lei
Zeng, Lingyuan
He, Dongdong
Wei, Xiaochun
Nutrition and degeneration of articular cartilage
title Nutrition and degeneration of articular cartilage
title_full Nutrition and degeneration of articular cartilage
title_fullStr Nutrition and degeneration of articular cartilage
title_full_unstemmed Nutrition and degeneration of articular cartilage
title_short Nutrition and degeneration of articular cartilage
title_sort nutrition and degeneration of articular cartilage
topic Experimental Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3717153/
https://www.ncbi.nlm.nih.gov/pubmed/22476522
http://dx.doi.org/10.1007/s00167-012-1977-7
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