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Nutrition and degeneration of articular cartilage
PURPOSE: To determine the importance of synovial fluid (SF) or subchondral bone marrow (BM) as nutrition sources in cartilage degeneration. METHODS: Ninety-five-month-old male rabbits were randomly divided into 5 groups according to sources of nutrition: SFBM-both; BM-only; SF-only; None-SFBM; and F...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3717153/ https://www.ncbi.nlm.nih.gov/pubmed/22476522 http://dx.doi.org/10.1007/s00167-012-1977-7 |
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author | Wang, Yuze Wei, Lei Zeng, Lingyuan He, Dongdong Wei, Xiaochun |
author_facet | Wang, Yuze Wei, Lei Zeng, Lingyuan He, Dongdong Wei, Xiaochun |
author_sort | Wang, Yuze |
collection | PubMed |
description | PURPOSE: To determine the importance of synovial fluid (SF) or subchondral bone marrow (BM) as nutrition sources in cartilage degeneration. METHODS: Ninety-five-month-old male rabbits were randomly divided into 5 groups according to sources of nutrition: SFBM-both; BM-only; SF-only; None-SFBM; and Free plug (unrestricted). Nutrition to 4-mm-diameter cylindrical osteochondral plugs created on the trochlea of the distal femurs was obstructed by Polyvinyl Chloride (PVC) cap. Cartilage changes were assessed after 4, 8, and 12 weeks by histology, immunohistochemistry, and real-time PCR. RESULTS: Cartilage in the BM-only group suffered the greatest damage, followed by the None-SFBM and SF-only groups. Apoptosis was increased in the BM-only and None-SFBM groups compared with others. Cartilage was significantly thinner at all time points in the BM-only and None-SFBM groups when compared with SFBM-both and Free plug, whereas in the SF-only group, this difference occurred after 8 weeks. Compared with SFBM-both and Free plug, expression of collagen II and aggrecan mRNAs in all groups was decreased but MMP-3 increased, respectively. CONCLUSION: Our data indicate that SF-derived nutrition is the dominant source of sustenance for adult cartilage structure and function. Cartilage damage is observed when the only nutrition source is the BM. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00167-012-1977-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3717153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-37171532013-07-23 Nutrition and degeneration of articular cartilage Wang, Yuze Wei, Lei Zeng, Lingyuan He, Dongdong Wei, Xiaochun Knee Surg Sports Traumatol Arthrosc Experimental Study PURPOSE: To determine the importance of synovial fluid (SF) or subchondral bone marrow (BM) as nutrition sources in cartilage degeneration. METHODS: Ninety-five-month-old male rabbits were randomly divided into 5 groups according to sources of nutrition: SFBM-both; BM-only; SF-only; None-SFBM; and Free plug (unrestricted). Nutrition to 4-mm-diameter cylindrical osteochondral plugs created on the trochlea of the distal femurs was obstructed by Polyvinyl Chloride (PVC) cap. Cartilage changes were assessed after 4, 8, and 12 weeks by histology, immunohistochemistry, and real-time PCR. RESULTS: Cartilage in the BM-only group suffered the greatest damage, followed by the None-SFBM and SF-only groups. Apoptosis was increased in the BM-only and None-SFBM groups compared with others. Cartilage was significantly thinner at all time points in the BM-only and None-SFBM groups when compared with SFBM-both and Free plug, whereas in the SF-only group, this difference occurred after 8 weeks. Compared with SFBM-both and Free plug, expression of collagen II and aggrecan mRNAs in all groups was decreased but MMP-3 increased, respectively. CONCLUSION: Our data indicate that SF-derived nutrition is the dominant source of sustenance for adult cartilage structure and function. Cartilage damage is observed when the only nutrition source is the BM. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00167-012-1977-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2012-04-04 2013 /pmc/articles/PMC3717153/ /pubmed/22476522 http://dx.doi.org/10.1007/s00167-012-1977-7 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Experimental Study Wang, Yuze Wei, Lei Zeng, Lingyuan He, Dongdong Wei, Xiaochun Nutrition and degeneration of articular cartilage |
title | Nutrition and degeneration of articular cartilage |
title_full | Nutrition and degeneration of articular cartilage |
title_fullStr | Nutrition and degeneration of articular cartilage |
title_full_unstemmed | Nutrition and degeneration of articular cartilage |
title_short | Nutrition and degeneration of articular cartilage |
title_sort | nutrition and degeneration of articular cartilage |
topic | Experimental Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3717153/ https://www.ncbi.nlm.nih.gov/pubmed/22476522 http://dx.doi.org/10.1007/s00167-012-1977-7 |
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