A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differen...

Descripción completa

Detalles Bibliográficos
Autores principales: Zou, Lijin, Luo, Yonglun, Chen, Muwan, Wang, Gang, Ding, Ming, Petersen, Charlotte Christie, Kang, Ran, Dagnaes-Hansen, Frederik, Zeng, Yuanlin, Lv, Nonghua, Ma, Qing, Le, Dang Q. S., Besenbacher, Flemming, Bolund, Lars, Jensen, Thomas G., Kjems, Jørgen, Pu, William T., Bünger, Cody
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718204/
https://www.ncbi.nlm.nih.gov/pubmed/23873182
http://dx.doi.org/10.1038/srep02243
_version_ 1782277754713014272
author Zou, Lijin
Luo, Yonglun
Chen, Muwan
Wang, Gang
Ding, Ming
Petersen, Charlotte Christie
Kang, Ran
Dagnaes-Hansen, Frederik
Zeng, Yuanlin
Lv, Nonghua
Ma, Qing
Le, Dang Q. S.
Besenbacher, Flemming
Bolund, Lars
Jensen, Thomas G.
Kjems, Jørgen
Pu, William T.
Bünger, Cody
author_facet Zou, Lijin
Luo, Yonglun
Chen, Muwan
Wang, Gang
Ding, Ming
Petersen, Charlotte Christie
Kang, Ran
Dagnaes-Hansen, Frederik
Zeng, Yuanlin
Lv, Nonghua
Ma, Qing
Le, Dang Q. S.
Besenbacher, Flemming
Bolund, Lars
Jensen, Thomas G.
Kjems, Jørgen
Pu, William T.
Bünger, Cody
author_sort Zou, Lijin
collection PubMed
description We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency – as seen with the loss of markers OCT3/4 and TRA-1-81 – and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.
format Online
Article
Text
id pubmed-3718204
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-37182042013-07-22 A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds Zou, Lijin Luo, Yonglun Chen, Muwan Wang, Gang Ding, Ming Petersen, Charlotte Christie Kang, Ran Dagnaes-Hansen, Frederik Zeng, Yuanlin Lv, Nonghua Ma, Qing Le, Dang Q. S. Besenbacher, Flemming Bolund, Lars Jensen, Thomas G. Kjems, Jørgen Pu, William T. Bünger, Cody Sci Rep Article We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency – as seen with the loss of markers OCT3/4 and TRA-1-81 – and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future. Nature Publishing Group 2013-07-22 /pmc/articles/PMC3718204/ /pubmed/23873182 http://dx.doi.org/10.1038/srep02243 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Zou, Lijin
Luo, Yonglun
Chen, Muwan
Wang, Gang
Ding, Ming
Petersen, Charlotte Christie
Kang, Ran
Dagnaes-Hansen, Frederik
Zeng, Yuanlin
Lv, Nonghua
Ma, Qing
Le, Dang Q. S.
Besenbacher, Flemming
Bolund, Lars
Jensen, Thomas G.
Kjems, Jørgen
Pu, William T.
Bünger, Cody
A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds
title A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds
title_full A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds
title_fullStr A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds
title_full_unstemmed A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds
title_short A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds
title_sort simple method for deriving functional mscs and applied for osteogenesis in 3d scaffolds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718204/
https://www.ncbi.nlm.nih.gov/pubmed/23873182
http://dx.doi.org/10.1038/srep02243
work_keys_str_mv AT zoulijin asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT luoyonglun asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT chenmuwan asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT wanggang asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT dingming asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT petersencharlottechristie asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT kangran asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT dagnaeshansenfrederik asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT zengyuanlin asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT lvnonghua asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT maqing asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT ledangqs asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT besenbacherflemming asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT bolundlars asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT jensenthomasg asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT kjemsjørgen asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT puwilliamt asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT bungercody asimplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT zoulijin simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT luoyonglun simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT chenmuwan simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT wanggang simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT dingming simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT petersencharlottechristie simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT kangran simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT dagnaeshansenfrederik simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT zengyuanlin simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT lvnonghua simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT maqing simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT ledangqs simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT besenbacherflemming simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT bolundlars simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT jensenthomasg simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT kjemsjørgen simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT puwilliamt simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds
AT bungercody simplemethodforderivingfunctionalmscsandappliedforosteogenesisin3dscaffolds

Ejemplares similares