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Cell therapy of pseudarthrosis

OBJECTIVE: To assess the safety and efficiency of cell therapy for pseudarthrosis. Implant of the bone marrow aspirate was compared to mononuclear cells purified extemporaneously using the Sepax(®) equipment. METHODS: Six patients with nonunion of the tibia or femur were treated. Four received a per...

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Autores principales: Bastos Filho, Ricardo, Lermontov, Simone, Borojevic, Radovan, Schott, Paulo Cezar, Gameiro, Vinicius Schott, Granjeiro, José Mauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Ortopedia e Traumatologia Regional de São Paulo 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718443/
https://www.ncbi.nlm.nih.gov/pubmed/24453616
http://dx.doi.org/10.1590/S1413-78522012000500005
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author Bastos Filho, Ricardo
Lermontov, Simone
Borojevic, Radovan
Schott, Paulo Cezar
Gameiro, Vinicius Schott
Granjeiro, José Mauro
author_facet Bastos Filho, Ricardo
Lermontov, Simone
Borojevic, Radovan
Schott, Paulo Cezar
Gameiro, Vinicius Schott
Granjeiro, José Mauro
author_sort Bastos Filho, Ricardo
collection PubMed
description OBJECTIVE: To assess the safety and efficiency of cell therapy for pseudarthrosis. Implant of the bone marrow aspirate was compared to mononuclear cells purified extemporaneously using the Sepax(®) equipment. METHODS: Six patients with nonunion of the tibia or femur were treated. Four received a percutaneous infusion of autologous bone marrow aspirated from the iliac crest, and two received autologous bone marrow mononuclear cells separated from the aspirate with the Sepax(®). The primary fixation method was unchanged, and the nonunion focus was not exposed. Physical examination and radiographies were performed 2, 4 and 6 months after the treatment by the same physician. After consolidation of the fracture the satisfaction of the patients was estimated using the adapted QALY scale. RESULTS: No complications occurred as a result of the referred procedures. Bone consolidation was obtained in all cases within 3 to 24 weeks. The degree of patient satisfaction before and after bone consolidation was assessed, with the average value increasing from two to nine (p=0.0156). CONCLUSION: We conclude that the proposed method is effective and safe for the treatment of nonunion of long bones regardless of the stabilization method used. Level of Evidence II, Prospective Comparative Study
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spelling pubmed-37184432014-01-22 Cell therapy of pseudarthrosis Bastos Filho, Ricardo Lermontov, Simone Borojevic, Radovan Schott, Paulo Cezar Gameiro, Vinicius Schott Granjeiro, José Mauro Acta Ortop Bras Original Article OBJECTIVE: To assess the safety and efficiency of cell therapy for pseudarthrosis. Implant of the bone marrow aspirate was compared to mononuclear cells purified extemporaneously using the Sepax(®) equipment. METHODS: Six patients with nonunion of the tibia or femur were treated. Four received a percutaneous infusion of autologous bone marrow aspirated from the iliac crest, and two received autologous bone marrow mononuclear cells separated from the aspirate with the Sepax(®). The primary fixation method was unchanged, and the nonunion focus was not exposed. Physical examination and radiographies were performed 2, 4 and 6 months after the treatment by the same physician. After consolidation of the fracture the satisfaction of the patients was estimated using the adapted QALY scale. RESULTS: No complications occurred as a result of the referred procedures. Bone consolidation was obtained in all cases within 3 to 24 weeks. The degree of patient satisfaction before and after bone consolidation was assessed, with the average value increasing from two to nine (p=0.0156). CONCLUSION: We conclude that the proposed method is effective and safe for the treatment of nonunion of long bones regardless of the stabilization method used. Level of Evidence II, Prospective Comparative Study Sociedade Brasileira de Ortopedia e Traumatologia Regional de São Paulo 2012 /pmc/articles/PMC3718443/ /pubmed/24453616 http://dx.doi.org/10.1590/S1413-78522012000500005 Text en http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License.
spellingShingle Original Article
Bastos Filho, Ricardo
Lermontov, Simone
Borojevic, Radovan
Schott, Paulo Cezar
Gameiro, Vinicius Schott
Granjeiro, José Mauro
Cell therapy of pseudarthrosis
title Cell therapy of pseudarthrosis
title_full Cell therapy of pseudarthrosis
title_fullStr Cell therapy of pseudarthrosis
title_full_unstemmed Cell therapy of pseudarthrosis
title_short Cell therapy of pseudarthrosis
title_sort cell therapy of pseudarthrosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718443/
https://www.ncbi.nlm.nih.gov/pubmed/24453616
http://dx.doi.org/10.1590/S1413-78522012000500005
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