Molecular identification of Giardia and Cryptosporidium from dogs and cats

The aim of the present study was to diagnose the presence of Giardia cysts and Cryptosporidium oocysts in household animals using nested polymerase chain reaction (PCR) and sequence analysis. One hundred faecal samples obtained from 81 dogs and 19 cats were investigated. The Cryptosporidium genotype...

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Autores principales: Sotiriadou, Isaia, Pantchev, Nikola, Gassmann, Doreen, Karanis, Panagiotis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718527/
https://www.ncbi.nlm.nih.gov/pubmed/23477297
http://dx.doi.org/10.1051/parasite/2013008
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author Sotiriadou, Isaia
Pantchev, Nikola
Gassmann, Doreen
Karanis, Panagiotis
author_facet Sotiriadou, Isaia
Pantchev, Nikola
Gassmann, Doreen
Karanis, Panagiotis
author_sort Sotiriadou, Isaia
collection PubMed
description The aim of the present study was to diagnose the presence of Giardia cysts and Cryptosporidium oocysts in household animals using nested polymerase chain reaction (PCR) and sequence analysis. One hundred faecal samples obtained from 81 dogs and 19 cats were investigated. The Cryptosporidium genotypes were determined by sequencing a fragment of the small subunit (SSU) rRNA gene, while the Giardia Assemblages were determined through analysis of the glutamate dehydrogenase (GDH) locus. Isolates from five dogs and two cats were positive by PCR for the presence of Giardia, and their sequences matched the zoonotic Assemblage A of Giardia. Cryptosporidium spp. isolated from one dog and one cat were both found to be C. parvum. One dog isolate harboured a mixed infection of C. parvum and Giardia Assemblage A. These findings support the growing evidence that household animals are potential reservoirs of the zoonotic pathogens Giardia spp. and Cryptosporidium spp. for infections in humans.
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spelling pubmed-37185272013-07-24 Molecular identification of Giardia and Cryptosporidium from dogs and cats Sotiriadou, Isaia Pantchev, Nikola Gassmann, Doreen Karanis, Panagiotis Parasite Research Article The aim of the present study was to diagnose the presence of Giardia cysts and Cryptosporidium oocysts in household animals using nested polymerase chain reaction (PCR) and sequence analysis. One hundred faecal samples obtained from 81 dogs and 19 cats were investigated. The Cryptosporidium genotypes were determined by sequencing a fragment of the small subunit (SSU) rRNA gene, while the Giardia Assemblages were determined through analysis of the glutamate dehydrogenase (GDH) locus. Isolates from five dogs and two cats were positive by PCR for the presence of Giardia, and their sequences matched the zoonotic Assemblage A of Giardia. Cryptosporidium spp. isolated from one dog and one cat were both found to be C. parvum. One dog isolate harboured a mixed infection of C. parvum and Giardia Assemblage A. These findings support the growing evidence that household animals are potential reservoirs of the zoonotic pathogens Giardia spp. and Cryptosporidium spp. for infections in humans. EDP Sciences 2013 2013-03-12 /pmc/articles/PMC3718527/ /pubmed/23477297 http://dx.doi.org/10.1051/parasite/2013008 Text en © I. Sotiriadou et al., published by EDP Sciences, 2013 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sotiriadou, Isaia
Pantchev, Nikola
Gassmann, Doreen
Karanis, Panagiotis
Molecular identification of Giardia and Cryptosporidium from dogs and cats
title Molecular identification of Giardia and Cryptosporidium from dogs and cats
title_full Molecular identification of Giardia and Cryptosporidium from dogs and cats
title_fullStr Molecular identification of Giardia and Cryptosporidium from dogs and cats
title_full_unstemmed Molecular identification of Giardia and Cryptosporidium from dogs and cats
title_short Molecular identification of Giardia and Cryptosporidium from dogs and cats
title_sort molecular identification of giardia and cryptosporidium from dogs and cats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718527/
https://www.ncbi.nlm.nih.gov/pubmed/23477297
http://dx.doi.org/10.1051/parasite/2013008
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