Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting

To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of Toxoplasma-specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of Toxoplasma...

Descripción completa

Detalles Bibliográficos
Autores principales: Ali-Heydari, Siamak, Keshavarz, Hossien, Shojaee, Saeedeh, Mohebali, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718541/
https://www.ncbi.nlm.nih.gov/pubmed/23688778
http://dx.doi.org/10.1051/parasite/2013017
_version_ 1782277788286320640
author Ali-Heydari, Siamak
Keshavarz, Hossien
Shojaee, Saeedeh
Mohebali, Mehdi
author_facet Ali-Heydari, Siamak
Keshavarz, Hossien
Shojaee, Saeedeh
Mohebali, Mehdi
author_sort Ali-Heydari, Siamak
collection PubMed
description To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of Toxoplasma-specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of Toxoplasma gondii infection according to IgG avidity ELISA. IgG avidity immunoblotting was performed, and antigenic bands with molecular weights of 22, 25, 28, 30, 32, 42, 44, 49, 55, 60, 66, 69, 88, 106, 130 and 157 kDa were recognized as low avidity markers. The most prevalent antigen for low avidity was p22. It is concluded that IgG avidity immunoblotting could distinguish acute and chronic phases of T. gondii infection.
format Online
Article
Text
id pubmed-3718541
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher EDP Sciences
record_format MEDLINE/PubMed
spelling pubmed-37185412013-07-24 Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting Ali-Heydari, Siamak Keshavarz, Hossien Shojaee, Saeedeh Mohebali, Mehdi Parasite Research Article To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of Toxoplasma-specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of Toxoplasma gondii infection according to IgG avidity ELISA. IgG avidity immunoblotting was performed, and antigenic bands with molecular weights of 22, 25, 28, 30, 32, 42, 44, 49, 55, 60, 66, 69, 88, 106, 130 and 157 kDa were recognized as low avidity markers. The most prevalent antigen for low avidity was p22. It is concluded that IgG avidity immunoblotting could distinguish acute and chronic phases of T. gondii infection. EDP Sciences 2013 2013-05-22 /pmc/articles/PMC3718541/ /pubmed/23688778 http://dx.doi.org/10.1051/parasite/2013017 Text en © S. Ali-Heydari et al., published by EDP Sciences, 2013 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ali-Heydari, Siamak
Keshavarz, Hossien
Shojaee, Saeedeh
Mohebali, Mehdi
Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting
title Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting
title_full Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting
title_fullStr Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting
title_full_unstemmed Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting
title_short Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting
title_sort diagnosis of antigenic markers of acute toxoplasmosis by igg avidity immunoblotting
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718541/
https://www.ncbi.nlm.nih.gov/pubmed/23688778
http://dx.doi.org/10.1051/parasite/2013017
work_keys_str_mv AT aliheydarisiamak diagnosisofantigenicmarkersofacutetoxoplasmosisbyiggavidityimmunoblotting
AT keshavarzhossien diagnosisofantigenicmarkersofacutetoxoplasmosisbyiggavidityimmunoblotting
AT shojaeesaeedeh diagnosisofantigenicmarkersofacutetoxoplasmosisbyiggavidityimmunoblotting
AT mohebalimehdi diagnosisofantigenicmarkersofacutetoxoplasmosisbyiggavidityimmunoblotting

Ejemplares similares