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Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes

Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans strain with phenotypic investigations of its important pathogenic features. We pr...

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Autores principales: Jakobsen, Tim Holm, Hansen, Martin Asser, Jensen, Peter Østrup, Hansen, Lars, Riber, Leise, Cockburn, April, Kolpen, Mette, Rønne Hansen, Christine, Ridderberg, Winnie, Eickhardt, Steffen, Hansen, Marlene, Kerpedjiev, Peter, Alhede, Morten, Qvortrup, Klaus, Burmølle, Mette, Moser, Claus, Kühl, Michael, Ciofu, Oana, Givskov, Michael, Sørensen, Søren J., Høiby, Niels, Bjarnsholt, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718787/
https://www.ncbi.nlm.nih.gov/pubmed/23894309
http://dx.doi.org/10.1371/journal.pone.0068484
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author Jakobsen, Tim Holm
Hansen, Martin Asser
Jensen, Peter Østrup
Hansen, Lars
Riber, Leise
Cockburn, April
Kolpen, Mette
Rønne Hansen, Christine
Ridderberg, Winnie
Eickhardt, Steffen
Hansen, Marlene
Kerpedjiev, Peter
Alhede, Morten
Qvortrup, Klaus
Burmølle, Mette
Moser, Claus
Kühl, Michael
Ciofu, Oana
Givskov, Michael
Sørensen, Søren J.
Høiby, Niels
Bjarnsholt, Thomas
author_facet Jakobsen, Tim Holm
Hansen, Martin Asser
Jensen, Peter Østrup
Hansen, Lars
Riber, Leise
Cockburn, April
Kolpen, Mette
Rønne Hansen, Christine
Ridderberg, Winnie
Eickhardt, Steffen
Hansen, Marlene
Kerpedjiev, Peter
Alhede, Morten
Qvortrup, Klaus
Burmølle, Mette
Moser, Claus
Kühl, Michael
Ciofu, Oana
Givskov, Michael
Sørensen, Søren J.
Høiby, Niels
Bjarnsholt, Thomas
author_sort Jakobsen, Tim Holm
collection PubMed
description Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of A. xylosoxidans NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of A. xylosoxidans NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of A. xylosoxidans and thereby improves our understanding and ability to treat this emerging pathogen.
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spelling pubmed-37187872013-07-26 Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes Jakobsen, Tim Holm Hansen, Martin Asser Jensen, Peter Østrup Hansen, Lars Riber, Leise Cockburn, April Kolpen, Mette Rønne Hansen, Christine Ridderberg, Winnie Eickhardt, Steffen Hansen, Marlene Kerpedjiev, Peter Alhede, Morten Qvortrup, Klaus Burmølle, Mette Moser, Claus Kühl, Michael Ciofu, Oana Givskov, Michael Sørensen, Søren J. Høiby, Niels Bjarnsholt, Thomas PLoS One Research Article Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of A. xylosoxidans NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of A. xylosoxidans NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of A. xylosoxidans and thereby improves our understanding and ability to treat this emerging pathogen. Public Library of Science 2013-07-22 /pmc/articles/PMC3718787/ /pubmed/23894309 http://dx.doi.org/10.1371/journal.pone.0068484 Text en © 2013 Jakobsen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jakobsen, Tim Holm
Hansen, Martin Asser
Jensen, Peter Østrup
Hansen, Lars
Riber, Leise
Cockburn, April
Kolpen, Mette
Rønne Hansen, Christine
Ridderberg, Winnie
Eickhardt, Steffen
Hansen, Marlene
Kerpedjiev, Peter
Alhede, Morten
Qvortrup, Klaus
Burmølle, Mette
Moser, Claus
Kühl, Michael
Ciofu, Oana
Givskov, Michael
Sørensen, Søren J.
Høiby, Niels
Bjarnsholt, Thomas
Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes
title Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes
title_full Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes
title_fullStr Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes
title_full_unstemmed Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes
title_short Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes
title_sort complete genome sequence of the cystic fibrosis pathogen achromobacter xylosoxidans nh44784-1996 complies with important pathogenic phenotypes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718787/
https://www.ncbi.nlm.nih.gov/pubmed/23894309
http://dx.doi.org/10.1371/journal.pone.0068484
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