Cargando…

Systematic characterization of the conformation and dynamics of budding yeast chromosome XII

Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII...

Descripción completa

Detalles Bibliográficos
Autores principales: Albert, Benjamin, Mathon, Julien, Shukla, Ashutosh, Saad, Hicham, Normand, Christophe, Léger-Silvestre, Isabelle, Villa, David, Kamgoue, Alain, Mozziconacci, Julien, Wong, Hua, Zimmer, Christophe, Bhargava, Purnima, Bancaud, Aurélien, Gadal, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718979/
https://www.ncbi.nlm.nih.gov/pubmed/23878273
http://dx.doi.org/10.1083/jcb.201208186
Descripción
Sumario:Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ∼100 kb along the chromosome, and investigated their motion over broad time scales (0.2–400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion.