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Systematic characterization of the conformation and dynamics of budding yeast chromosome XII

Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII...

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Autores principales: Albert, Benjamin, Mathon, Julien, Shukla, Ashutosh, Saad, Hicham, Normand, Christophe, Léger-Silvestre, Isabelle, Villa, David, Kamgoue, Alain, Mozziconacci, Julien, Wong, Hua, Zimmer, Christophe, Bhargava, Purnima, Bancaud, Aurélien, Gadal, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718979/
https://www.ncbi.nlm.nih.gov/pubmed/23878273
http://dx.doi.org/10.1083/jcb.201208186
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author Albert, Benjamin
Mathon, Julien
Shukla, Ashutosh
Saad, Hicham
Normand, Christophe
Léger-Silvestre, Isabelle
Villa, David
Kamgoue, Alain
Mozziconacci, Julien
Wong, Hua
Zimmer, Christophe
Bhargava, Purnima
Bancaud, Aurélien
Gadal, Olivier
author_facet Albert, Benjamin
Mathon, Julien
Shukla, Ashutosh
Saad, Hicham
Normand, Christophe
Léger-Silvestre, Isabelle
Villa, David
Kamgoue, Alain
Mozziconacci, Julien
Wong, Hua
Zimmer, Christophe
Bhargava, Purnima
Bancaud, Aurélien
Gadal, Olivier
author_sort Albert, Benjamin
collection PubMed
description Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ∼100 kb along the chromosome, and investigated their motion over broad time scales (0.2–400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion.
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spelling pubmed-37189792014-01-22 Systematic characterization of the conformation and dynamics of budding yeast chromosome XII Albert, Benjamin Mathon, Julien Shukla, Ashutosh Saad, Hicham Normand, Christophe Léger-Silvestre, Isabelle Villa, David Kamgoue, Alain Mozziconacci, Julien Wong, Hua Zimmer, Christophe Bhargava, Purnima Bancaud, Aurélien Gadal, Olivier J Cell Biol Research Articles Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ∼100 kb along the chromosome, and investigated their motion over broad time scales (0.2–400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion. The Rockefeller University Press 2013-07-22 /pmc/articles/PMC3718979/ /pubmed/23878273 http://dx.doi.org/10.1083/jcb.201208186 Text en © 2013 Albert et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Albert, Benjamin
Mathon, Julien
Shukla, Ashutosh
Saad, Hicham
Normand, Christophe
Léger-Silvestre, Isabelle
Villa, David
Kamgoue, Alain
Mozziconacci, Julien
Wong, Hua
Zimmer, Christophe
Bhargava, Purnima
Bancaud, Aurélien
Gadal, Olivier
Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
title Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
title_full Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
title_fullStr Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
title_full_unstemmed Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
title_short Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
title_sort systematic characterization of the conformation and dynamics of budding yeast chromosome xii
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718979/
https://www.ncbi.nlm.nih.gov/pubmed/23878273
http://dx.doi.org/10.1083/jcb.201208186
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