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Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718979/ https://www.ncbi.nlm.nih.gov/pubmed/23878273 http://dx.doi.org/10.1083/jcb.201208186 |
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author | Albert, Benjamin Mathon, Julien Shukla, Ashutosh Saad, Hicham Normand, Christophe Léger-Silvestre, Isabelle Villa, David Kamgoue, Alain Mozziconacci, Julien Wong, Hua Zimmer, Christophe Bhargava, Purnima Bancaud, Aurélien Gadal, Olivier |
author_facet | Albert, Benjamin Mathon, Julien Shukla, Ashutosh Saad, Hicham Normand, Christophe Léger-Silvestre, Isabelle Villa, David Kamgoue, Alain Mozziconacci, Julien Wong, Hua Zimmer, Christophe Bhargava, Purnima Bancaud, Aurélien Gadal, Olivier |
author_sort | Albert, Benjamin |
collection | PubMed |
description | Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ∼100 kb along the chromosome, and investigated their motion over broad time scales (0.2–400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion. |
format | Online Article Text |
id | pubmed-3718979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-37189792014-01-22 Systematic characterization of the conformation and dynamics of budding yeast chromosome XII Albert, Benjamin Mathon, Julien Shukla, Ashutosh Saad, Hicham Normand, Christophe Léger-Silvestre, Isabelle Villa, David Kamgoue, Alain Mozziconacci, Julien Wong, Hua Zimmer, Christophe Bhargava, Purnima Bancaud, Aurélien Gadal, Olivier J Cell Biol Research Articles Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ∼100 kb along the chromosome, and investigated their motion over broad time scales (0.2–400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion. The Rockefeller University Press 2013-07-22 /pmc/articles/PMC3718979/ /pubmed/23878273 http://dx.doi.org/10.1083/jcb.201208186 Text en © 2013 Albert et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Albert, Benjamin Mathon, Julien Shukla, Ashutosh Saad, Hicham Normand, Christophe Léger-Silvestre, Isabelle Villa, David Kamgoue, Alain Mozziconacci, Julien Wong, Hua Zimmer, Christophe Bhargava, Purnima Bancaud, Aurélien Gadal, Olivier Systematic characterization of the conformation and dynamics of budding yeast chromosome XII |
title | Systematic characterization of the conformation and dynamics of budding yeast chromosome XII |
title_full | Systematic characterization of the conformation and dynamics of budding yeast chromosome XII |
title_fullStr | Systematic characterization of the conformation and dynamics of budding yeast chromosome XII |
title_full_unstemmed | Systematic characterization of the conformation and dynamics of budding yeast chromosome XII |
title_short | Systematic characterization of the conformation and dynamics of budding yeast chromosome XII |
title_sort | systematic characterization of the conformation and dynamics of budding yeast chromosome xii |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718979/ https://www.ncbi.nlm.nih.gov/pubmed/23878273 http://dx.doi.org/10.1083/jcb.201208186 |
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