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Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study

INTRODUCTION: The cells expressing Indoleamine 2, 3-dioxygenase (IDO) in feto-maternal interface mediate tryptophan catabolism, hence protect allogeneic fetus from lethal rejection by maternal immune responses. In this study, we report immuno-localization of IDO(+) cells in murine reproductive tract...

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Autores principales: Hemmati, Shayda, Jeddi-Tehrani, Mahmood, Torkabadi, Ebrahim, Ghassemi, Jamileh, Kazemi sefat, Golnaz Ensieh, Danesh, Parivash, Barzegar Yarmohammadi, Leila, Akhondi, Mohammad Mehdi, Zarnani, Amir Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719323/
https://www.ncbi.nlm.nih.gov/pubmed/23926466
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author Hemmati, Shayda
Jeddi-Tehrani, Mahmood
Torkabadi, Ebrahim
Ghassemi, Jamileh
Kazemi sefat, Golnaz Ensieh
Danesh, Parivash
Barzegar Yarmohammadi, Leila
Akhondi, Mohammad Mehdi
Zarnani, Amir Hassan
author_facet Hemmati, Shayda
Jeddi-Tehrani, Mahmood
Torkabadi, Ebrahim
Ghassemi, Jamileh
Kazemi sefat, Golnaz Ensieh
Danesh, Parivash
Barzegar Yarmohammadi, Leila
Akhondi, Mohammad Mehdi
Zarnani, Amir Hassan
author_sort Hemmati, Shayda
collection PubMed
description INTRODUCTION: The cells expressing Indoleamine 2, 3-dioxygenase (IDO) in feto-maternal interface mediate tryptophan catabolism, hence protect allogeneic fetus from lethal rejection by maternal immune responses. In this study, we report immuno-localization of IDO(+) cells in murine reproductive tract and placenta throughout mouse pregnancy by immunohistochemistry. MATERIALS AND METHODS: Syngeneic pregnant mice were examined for vaginal plug to discover about their state of pregnancy. A total of three pregnant mice were examined at each stage.The examination was further confirmed by the detection of sperm in vaginal smear. On the gestational days of 2(nd), 12(th) and 18(th), the uterus and oviduct were removed and expression of IDO was investigated in the endometrium, placenta and oviduct by immunohistochemistry. RESULTS: Our results showed that IDO is expressed consistently in feto-maternal interface throughout pregnancy. In endometrium, expression of IDO was predominantly confined to luminal and glandular epithelial cells. Cells at junctional and labyrinth zones of placenta showed strong IDO immunoreactivity as well. CONCLUSION: Expression of IDO at the protein level in reproductive tract of pregnant mice during entire periods of gestation points to its potential protective role in maintenance of pregnancy. In our knowledge this is the first report of expression of IDO in feto-maternal phase during murine pregnancy.
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spelling pubmed-37193232013-08-07 Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study Hemmati, Shayda Jeddi-Tehrani, Mahmood Torkabadi, Ebrahim Ghassemi, Jamileh Kazemi sefat, Golnaz Ensieh Danesh, Parivash Barzegar Yarmohammadi, Leila Akhondi, Mohammad Mehdi Zarnani, Amir Hassan J Reprod Infertil Original Article INTRODUCTION: The cells expressing Indoleamine 2, 3-dioxygenase (IDO) in feto-maternal interface mediate tryptophan catabolism, hence protect allogeneic fetus from lethal rejection by maternal immune responses. In this study, we report immuno-localization of IDO(+) cells in murine reproductive tract and placenta throughout mouse pregnancy by immunohistochemistry. MATERIALS AND METHODS: Syngeneic pregnant mice were examined for vaginal plug to discover about their state of pregnancy. A total of three pregnant mice were examined at each stage.The examination was further confirmed by the detection of sperm in vaginal smear. On the gestational days of 2(nd), 12(th) and 18(th), the uterus and oviduct were removed and expression of IDO was investigated in the endometrium, placenta and oviduct by immunohistochemistry. RESULTS: Our results showed that IDO is expressed consistently in feto-maternal interface throughout pregnancy. In endometrium, expression of IDO was predominantly confined to luminal and glandular epithelial cells. Cells at junctional and labyrinth zones of placenta showed strong IDO immunoreactivity as well. CONCLUSION: Expression of IDO at the protein level in reproductive tract of pregnant mice during entire periods of gestation points to its potential protective role in maintenance of pregnancy. In our knowledge this is the first report of expression of IDO in feto-maternal phase during murine pregnancy. Avicenna Research Institute 2009 /pmc/articles/PMC3719323/ /pubmed/23926466 Text en Copyright © 2009 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Hemmati, Shayda
Jeddi-Tehrani, Mahmood
Torkabadi, Ebrahim
Ghassemi, Jamileh
Kazemi sefat, Golnaz Ensieh
Danesh, Parivash
Barzegar Yarmohammadi, Leila
Akhondi, Mohammad Mehdi
Zarnani, Amir Hassan
Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study
title Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study
title_full Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study
title_fullStr Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study
title_full_unstemmed Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study
title_short Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study
title_sort indoleamine 2,3-dioxygenase (ido) is expressed at feto-placental unit throughout mouse gestation: an immunohistochemical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719323/
https://www.ncbi.nlm.nih.gov/pubmed/23926466
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