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A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning

INTRODUCTION: PERF15 is a testicular germ-cell specific fatty-acid binding protein (FABP) isolated from mammals, originally from rats. It encodes one of the most abundant proteins of rat spermatozoa localized in the perinuclear theca. Northern blot analysis has demonstrated that rat PERF15 mRNA is e...

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Autores principales: Pouresmaeili, Farkhondeh, Khalili, Tahereh, Jeddi-Tehrani, Mahmood, Bandehpour, Mojgan, Ghasemi, Jamileh, Salehi-Niya, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719324/
https://www.ncbi.nlm.nih.gov/pubmed/23926469
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author Pouresmaeili, Farkhondeh
Khalili, Tahereh
Jeddi-Tehrani, Mahmood
Bandehpour, Mojgan
Ghasemi, Jamileh
Salehi-Niya, Fatemeh
author_facet Pouresmaeili, Farkhondeh
Khalili, Tahereh
Jeddi-Tehrani, Mahmood
Bandehpour, Mojgan
Ghasemi, Jamileh
Salehi-Niya, Fatemeh
author_sort Pouresmaeili, Farkhondeh
collection PubMed
description INTRODUCTION: PERF15 is a testicular germ-cell specific fatty-acid binding protein (FABP) isolated from mammals, originally from rats. It encodes one of the most abundant proteins of rat spermatozoa localized in the perinuclear theca. Northern blot analysis has demonstrated that rat PERF15 mRNA is exclusively transcribed during meiosis and post-meiosis. In this study, we cloned and sequenced human PERF15 gene. MATERIALS AND METHODS: According to the open reading frame of automated computational analysis of Homo sapiens similar to testis fatty acid binding protein nine, two specific Primers were designed to amplify human PERF15 gene. To confirm the identity of the amplified gene, PCR products of PERF15 were cloned into appropriate plasmid vectors followed by sequencing of the inserts. RESULTS: A unique band of ∼3kb was obtained after PCR amplification. Restriction enzyme digestion using PvuII confirmed that the fragment was related to PERF15. Gene alignment, direct sequencing and application of specific primers to the gene showed 100% similarity between this gene and the computational data by gel extraction of the ∼3 kb band. The human PERF15 gene contained four exons and three introns. Exons one, two, three and four, respectively, coded for 24, 57, 34 and 17 amino acids. The existing three introns were composed of 2113, 461, and 168 nucleotides. CONCLUSION: In spite of the homology between exonic regions and exon-intron boundaries of human PERF15 gene and that of animals, human PERF15 gene is different in size and sequence from corresponding introns in rat and murine PERF15.
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spelling pubmed-37193242013-08-07 A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning Pouresmaeili, Farkhondeh Khalili, Tahereh Jeddi-Tehrani, Mahmood Bandehpour, Mojgan Ghasemi, Jamileh Salehi-Niya, Fatemeh J Reprod Infertil Original Article INTRODUCTION: PERF15 is a testicular germ-cell specific fatty-acid binding protein (FABP) isolated from mammals, originally from rats. It encodes one of the most abundant proteins of rat spermatozoa localized in the perinuclear theca. Northern blot analysis has demonstrated that rat PERF15 mRNA is exclusively transcribed during meiosis and post-meiosis. In this study, we cloned and sequenced human PERF15 gene. MATERIALS AND METHODS: According to the open reading frame of automated computational analysis of Homo sapiens similar to testis fatty acid binding protein nine, two specific Primers were designed to amplify human PERF15 gene. To confirm the identity of the amplified gene, PCR products of PERF15 were cloned into appropriate plasmid vectors followed by sequencing of the inserts. RESULTS: A unique band of ∼3kb was obtained after PCR amplification. Restriction enzyme digestion using PvuII confirmed that the fragment was related to PERF15. Gene alignment, direct sequencing and application of specific primers to the gene showed 100% similarity between this gene and the computational data by gel extraction of the ∼3 kb band. The human PERF15 gene contained four exons and three introns. Exons one, two, three and four, respectively, coded for 24, 57, 34 and 17 amino acids. The existing three introns were composed of 2113, 461, and 168 nucleotides. CONCLUSION: In spite of the homology between exonic regions and exon-intron boundaries of human PERF15 gene and that of animals, human PERF15 gene is different in size and sequence from corresponding introns in rat and murine PERF15. Avicenna Research Institute 2009 /pmc/articles/PMC3719324/ /pubmed/23926469 Text en Copyright © 2009 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Pouresmaeili, Farkhondeh
Khalili, Tahereh
Jeddi-Tehrani, Mahmood
Bandehpour, Mojgan
Ghasemi, Jamileh
Salehi-Niya, Fatemeh
A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning
title A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning
title_full A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning
title_fullStr A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning
title_full_unstemmed A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning
title_short A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning
title_sort novel human lipid binding protein coding gene: perf15, sequence and cloning
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719324/
https://www.ncbi.nlm.nih.gov/pubmed/23926469
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