Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus

The emergence of mecC methicillin-resistant Staphylococcus aureus (MRSA) poses a diagnostic challenge for clinical microbiology laboratories. Using the Vitek 2 system, we tested a panel of 896 Staphylococcus aureus isolates and found that an oxacillin-sensitive/cefoxitin-resistant profile had a sens...

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Autores principales: Cartwright, Edward J. P., Paterson, Gavin K., Raven, Kathy E., Harrison, Ewan M., Gouliouris, Theodore, Kearns, Angela, Pichon, Bruno, Edwards, Giles, Skov, Robert L., Larsen, Anders R., Holmes, Mark A., Parkhill, Julian, Peacock, Sharon J., Török, M. Estée
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719650/
https://www.ncbi.nlm.nih.gov/pubmed/23720794
http://dx.doi.org/10.1128/JCM.00847-13
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author Cartwright, Edward J. P.
Paterson, Gavin K.
Raven, Kathy E.
Harrison, Ewan M.
Gouliouris, Theodore
Kearns, Angela
Pichon, Bruno
Edwards, Giles
Skov, Robert L.
Larsen, Anders R.
Holmes, Mark A.
Parkhill, Julian
Peacock, Sharon J.
Török, M. Estée
author_facet Cartwright, Edward J. P.
Paterson, Gavin K.
Raven, Kathy E.
Harrison, Ewan M.
Gouliouris, Theodore
Kearns, Angela
Pichon, Bruno
Edwards, Giles
Skov, Robert L.
Larsen, Anders R.
Holmes, Mark A.
Parkhill, Julian
Peacock, Sharon J.
Török, M. Estée
author_sort Cartwright, Edward J. P.
collection PubMed
description The emergence of mecC methicillin-resistant Staphylococcus aureus (MRSA) poses a diagnostic challenge for clinical microbiology laboratories. Using the Vitek 2 system, we tested a panel of 896 Staphylococcus aureus isolates and found that an oxacillin-sensitive/cefoxitin-resistant profile had a sensitivity of 88.7% and a specificity of 99.5% for the identification of mecC MRSA isolates. The presence of the mecC gene, determined by bacterial whole-genome sequencing, was used as the gold standard. This profile could provide a zero-cost screening method for identification of mecC-positive MRSA strains.
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spelling pubmed-37196502013-08-03 Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus Cartwright, Edward J. P. Paterson, Gavin K. Raven, Kathy E. Harrison, Ewan M. Gouliouris, Theodore Kearns, Angela Pichon, Bruno Edwards, Giles Skov, Robert L. Larsen, Anders R. Holmes, Mark A. Parkhill, Julian Peacock, Sharon J. Török, M. Estée J Clin Microbiol Bacteriology The emergence of mecC methicillin-resistant Staphylococcus aureus (MRSA) poses a diagnostic challenge for clinical microbiology laboratories. Using the Vitek 2 system, we tested a panel of 896 Staphylococcus aureus isolates and found that an oxacillin-sensitive/cefoxitin-resistant profile had a sensitivity of 88.7% and a specificity of 99.5% for the identification of mecC MRSA isolates. The presence of the mecC gene, determined by bacterial whole-genome sequencing, was used as the gold standard. This profile could provide a zero-cost screening method for identification of mecC-positive MRSA strains. American Society for Microbiology 2013-08 /pmc/articles/PMC3719650/ /pubmed/23720794 http://dx.doi.org/10.1128/JCM.00847-13 Text en Copyright © 2013 Cartwright et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Bacteriology
Cartwright, Edward J. P.
Paterson, Gavin K.
Raven, Kathy E.
Harrison, Ewan M.
Gouliouris, Theodore
Kearns, Angela
Pichon, Bruno
Edwards, Giles
Skov, Robert L.
Larsen, Anders R.
Holmes, Mark A.
Parkhill, Julian
Peacock, Sharon J.
Török, M. Estée
Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
title Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
title_full Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
title_fullStr Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
title_short Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
title_sort use of vitek 2 antimicrobial susceptibility profile to identify mecc in methicillin-resistant staphylococcus aureus
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719650/
https://www.ncbi.nlm.nih.gov/pubmed/23720794
http://dx.doi.org/10.1128/JCM.00847-13
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