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A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties
Adhesion to digestive mucosa is considered a crucial first step in the pathogenicity of invasive Candida infections. Candida glabrata disseminated infections predominantly start from the gut. A mouse model of disseminated infection starting from the gut was set up. Hematogenous dissemination was obt...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720583/ https://www.ncbi.nlm.nih.gov/pubmed/23936069 http://dx.doi.org/10.1371/journal.pone.0069664 |
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author | Atanasova, Ralitsa Angoulvant, Adela Tefit, Maurel Gay, Frédérick Guitard, Juliette Mazier, Dominique Fairhead, Cécile Hennequin, Christophe |
author_facet | Atanasova, Ralitsa Angoulvant, Adela Tefit, Maurel Gay, Frédérick Guitard, Juliette Mazier, Dominique Fairhead, Cécile Hennequin, Christophe |
author_sort | Atanasova, Ralitsa |
collection | PubMed |
description | Adhesion to digestive mucosa is considered a crucial first step in the pathogenicity of invasive Candida infections. Candida glabrata disseminated infections predominantly start from the gut. A mouse model of disseminated infection starting from the gut was set up. Hematogenous dissemination was obtained after a low-protein diet followed by a regimen of cyclophosphamide-methotrexate and an oral inoculation of the yeasts via the drinking water. The liver was the first organ infected (day 7 post-infection), and lethality was 100% at day 21 post-infection. This new mouse model was used to compare the mortality rate and fungal burden in deep organs induced by 5 strains exhibiting different levels of adhesion to enterocyte Caco-2 cells, as determined in a test on 36 C. glabrata strains. In this model, no statistical difference of lethality was demonstrated between the strains, and fungal burden varied in kidneys and lungs but without correlation with the level of adhesion to enterocytes. Further studies using the model developed here allow analysis of the crossing of the digestive mucosa by yeasts, and help relate this to yet-poorly understood adhesion phenotypes. |
format | Online Article Text |
id | pubmed-3720583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37205832013-08-09 A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties Atanasova, Ralitsa Angoulvant, Adela Tefit, Maurel Gay, Frédérick Guitard, Juliette Mazier, Dominique Fairhead, Cécile Hennequin, Christophe PLoS One Research Article Adhesion to digestive mucosa is considered a crucial first step in the pathogenicity of invasive Candida infections. Candida glabrata disseminated infections predominantly start from the gut. A mouse model of disseminated infection starting from the gut was set up. Hematogenous dissemination was obtained after a low-protein diet followed by a regimen of cyclophosphamide-methotrexate and an oral inoculation of the yeasts via the drinking water. The liver was the first organ infected (day 7 post-infection), and lethality was 100% at day 21 post-infection. This new mouse model was used to compare the mortality rate and fungal burden in deep organs induced by 5 strains exhibiting different levels of adhesion to enterocyte Caco-2 cells, as determined in a test on 36 C. glabrata strains. In this model, no statistical difference of lethality was demonstrated between the strains, and fungal burden varied in kidneys and lungs but without correlation with the level of adhesion to enterocytes. Further studies using the model developed here allow analysis of the crossing of the digestive mucosa by yeasts, and help relate this to yet-poorly understood adhesion phenotypes. Public Library of Science 2013-07-23 /pmc/articles/PMC3720583/ /pubmed/23936069 http://dx.doi.org/10.1371/journal.pone.0069664 Text en © 2013 Atanasova et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Atanasova, Ralitsa Angoulvant, Adela Tefit, Maurel Gay, Frédérick Guitard, Juliette Mazier, Dominique Fairhead, Cécile Hennequin, Christophe A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties |
title | A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties |
title_full | A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties |
title_fullStr | A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties |
title_full_unstemmed | A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties |
title_short | A Mouse Model for Candida glabrata Hematogenous Disseminated Infection Starting from the Gut: Evaluation of Strains with Different Adhesion Properties |
title_sort | mouse model for candida glabrata hematogenous disseminated infection starting from the gut: evaluation of strains with different adhesion properties |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720583/ https://www.ncbi.nlm.nih.gov/pubmed/23936069 http://dx.doi.org/10.1371/journal.pone.0069664 |
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