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Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma
BACKGROUND: Glioblastoma (GBM) confers a dismal prognosis despite advances in current therapy. Cancer-testis antigens (CTA) comprise families of tumor-associated antigens that are immunogenic in different cancers. The aim of this study was to determine the expression profile of a large number of CTA...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720610/ https://www.ncbi.nlm.nih.gov/pubmed/23592437 |
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author | Freitas, Marcelo Roberto Pereira Malheiros, Suzana Maria Fleury Stávale, João Norberto Biassi, Thais Priscila Zamunér, Fernando Tadeu de Souza Begnami, Maria Dirlei Ferreira Soares, Fernando Augusto Vettore, Andre Luíz |
author_facet | Freitas, Marcelo Roberto Pereira Malheiros, Suzana Maria Fleury Stávale, João Norberto Biassi, Thais Priscila Zamunér, Fernando Tadeu de Souza Begnami, Maria Dirlei Ferreira Soares, Fernando Augusto Vettore, Andre Luíz |
author_sort | Freitas, Marcelo Roberto Pereira |
collection | PubMed |
description | BACKGROUND: Glioblastoma (GBM) confers a dismal prognosis despite advances in current therapy. Cancer-testis antigens (CTA) comprise families of tumor-associated antigens that are immunogenic in different cancers. The aim of this study was to determine the expression profile of a large number of CTA genes in GBM. METHODS: We selected, from 153 CTA genes, those genes potentially expressed in GBM. The expression pattern of 30 CTA was then evaluated by RT-PCR in a series of 48 GBM and 5 normal brain samples. The presence of CTCFL protein was also evaluated by immunohistochemical staining. RESULTS: Among the genes with no expression in normal brain, ACTL8 (57%), OIP5 (54%), XAGE3 (44%) and CTCFL (15%) were frequently expressed in GBM, while over 85% of the tumors expressed at least 1 of these four CTA. Coexpression of two or more CTA occurred in 49% of cases. CTCFL protein expression was detected in 13% of the GBM and was negative in normal brain samples. GBM expressing 3-4 CTA was associated with significantly better overall survival (OS) rates (P = 0.017). By multivariate analysis, mRNA positivity for 3-4 CTA (P = 0.044), radiotherapy (P = 0.010) and chemotherapy (P = 0.001) were independent prognostic factors for OS. CONCLUSIONS: GBM frequently express ACTL8, OIP5, XAGE3 and CTCFL. A relatively high percentage of tumors expressed at least one of these four CTA, opening the perspective for their utility in antigen-specific immunotherapy. Furthermore, mRNA positivity for 3-4 CTA is an independent predictor of better OS for GBM patients. |
format | Online Article Text |
id | pubmed-3720610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-37206102013-07-30 Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma Freitas, Marcelo Roberto Pereira Malheiros, Suzana Maria Fleury Stávale, João Norberto Biassi, Thais Priscila Zamunér, Fernando Tadeu de Souza Begnami, Maria Dirlei Ferreira Soares, Fernando Augusto Vettore, Andre Luíz Oncotarget Research Paper BACKGROUND: Glioblastoma (GBM) confers a dismal prognosis despite advances in current therapy. Cancer-testis antigens (CTA) comprise families of tumor-associated antigens that are immunogenic in different cancers. The aim of this study was to determine the expression profile of a large number of CTA genes in GBM. METHODS: We selected, from 153 CTA genes, those genes potentially expressed in GBM. The expression pattern of 30 CTA was then evaluated by RT-PCR in a series of 48 GBM and 5 normal brain samples. The presence of CTCFL protein was also evaluated by immunohistochemical staining. RESULTS: Among the genes with no expression in normal brain, ACTL8 (57%), OIP5 (54%), XAGE3 (44%) and CTCFL (15%) were frequently expressed in GBM, while over 85% of the tumors expressed at least 1 of these four CTA. Coexpression of two or more CTA occurred in 49% of cases. CTCFL protein expression was detected in 13% of the GBM and was negative in normal brain samples. GBM expressing 3-4 CTA was associated with significantly better overall survival (OS) rates (P = 0.017). By multivariate analysis, mRNA positivity for 3-4 CTA (P = 0.044), radiotherapy (P = 0.010) and chemotherapy (P = 0.001) were independent prognostic factors for OS. CONCLUSIONS: GBM frequently express ACTL8, OIP5, XAGE3 and CTCFL. A relatively high percentage of tumors expressed at least one of these four CTA, opening the perspective for their utility in antigen-specific immunotherapy. Furthermore, mRNA positivity for 3-4 CTA is an independent predictor of better OS for GBM patients. Impact Journals LLC 2013-04-15 /pmc/articles/PMC3720610/ /pubmed/23592437 Text en Copyright: © 2013 Freitas et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
spellingShingle | Research Paper Freitas, Marcelo Roberto Pereira Malheiros, Suzana Maria Fleury Stávale, João Norberto Biassi, Thais Priscila Zamunér, Fernando Tadeu de Souza Begnami, Maria Dirlei Ferreira Soares, Fernando Augusto Vettore, Andre Luíz Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma |
title | Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma |
title_full | Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma |
title_fullStr | Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma |
title_full_unstemmed | Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma |
title_short | Expression of Cancer/Testis Antigens is Correlated with Improved Survival in Glioblastoma |
title_sort | expression of cancer/testis antigens is correlated with improved survival in glioblastoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720610/ https://www.ncbi.nlm.nih.gov/pubmed/23592437 |
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