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Diagnosis of Barmah Forest Virus Infection by a Nested Real-Time SYBR Green RT-PCR Assay

Barmah Forest virus (BFV) is a mosquito borne (+) ssRNA alphavirus found only in Australia. It causes rash, myalgia and arthralgia in humans and is usually diagnosed serologically. We developed a real-time PCR assay to detect BFV in an effort to improve diagnosis early in the course of infection. Th...

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Detalles Bibliográficos
Autores principales: Hueston, Linda, Toi, Cheryl S., Jeoffreys, Neisha, Sorrell, Tania, Gilbert, Gwendolyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720699/
https://www.ncbi.nlm.nih.gov/pubmed/23935816
http://dx.doi.org/10.1371/journal.pone.0065197