A Serine-Substituted P450 Catalyzes Highly Efficient Carbene Transfer to Olefins In Vivo

Genetically encoded catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a unique serine-heme ligated cytochrome “P411” that catalyzes efficient and selective carbene transfers from diazoesters to olefins in intact Escherichia coli cel...

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Detalles Bibliográficos
Autores principales: Coelho, Pedro S., Wang, Z. Jane, Ener, Maraia E., Baril, Stefanie A., Kannan, Arvind A., Arnold, Frances H., Brustad, Eric M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720782/
https://www.ncbi.nlm.nih.gov/pubmed/23792734
http://dx.doi.org/10.1038/nchembio.1278
Descripción
Sumario:Genetically encoded catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a unique serine-heme ligated cytochrome “P411” that catalyzes efficient and selective carbene transfers from diazoesters to olefins in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous-CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting state Fe(III/II) reduction potential, and significantly improves NAD(P)H-driven cyclopropanation activity.

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