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A simple implantation method for flexible, multisite microelectrodes into rat brains
A long term functional and reliable coupling between neural tissue and implanted microelectrodes is the key issue in acquiring neural electrophysiological signals or therapeutically excite neural tissue. The currently often used rigid micro-electrodes are thought to cause a severe foreign body react...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3721086/ https://www.ncbi.nlm.nih.gov/pubmed/23898266 http://dx.doi.org/10.3389/fneng.2013.00006 |
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author | Richter, Anja Xie, Yijing Schumacher, Anett Löffler, Susanne Kirch, Robert D. Al-Hasani, Jaafar Rapoport, Daniel H. Kruse, Charli Moser, Andreas Tronnier, Volker Danner, Sandra Hofmann, Ulrich G. |
author_facet | Richter, Anja Xie, Yijing Schumacher, Anett Löffler, Susanne Kirch, Robert D. Al-Hasani, Jaafar Rapoport, Daniel H. Kruse, Charli Moser, Andreas Tronnier, Volker Danner, Sandra Hofmann, Ulrich G. |
author_sort | Richter, Anja |
collection | PubMed |
description | A long term functional and reliable coupling between neural tissue and implanted microelectrodes is the key issue in acquiring neural electrophysiological signals or therapeutically excite neural tissue. The currently often used rigid micro-electrodes are thought to cause a severe foreign body reaction resulting in a thick glial scar and consequently a poor tissue-electrode coupling in the chronic phase. We hypothesize, that this adverse effect might be remedied by probes compliant to the soft brain tissue, i.e., replacing rigid electrodes by flexible ones. Unfortunately, this flexibility comes at the price of a low stiffness, which makes targeted low trauma implantation very challenging. In this study, we demonstrate an adaptable and simple method to implant extremely flexible microprobes even to deep areas of rat's brain. Implantation of flexible probes is achieved by rod supported stereotactic insertion fostered by a hydrogel (2% agarose in PBS) cushion on the exposed skull. We were thus able to implant very flexible micro-probes in 70 rats as deep as the rodent's subthalamic nucleus. This work describes in detail the procedures and steps needed for minimal invasive, but reliable implantation of flexible probes. |
format | Online Article Text |
id | pubmed-3721086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-37210862013-07-29 A simple implantation method for flexible, multisite microelectrodes into rat brains Richter, Anja Xie, Yijing Schumacher, Anett Löffler, Susanne Kirch, Robert D. Al-Hasani, Jaafar Rapoport, Daniel H. Kruse, Charli Moser, Andreas Tronnier, Volker Danner, Sandra Hofmann, Ulrich G. Front Neuroeng Neuroscience A long term functional and reliable coupling between neural tissue and implanted microelectrodes is the key issue in acquiring neural electrophysiological signals or therapeutically excite neural tissue. The currently often used rigid micro-electrodes are thought to cause a severe foreign body reaction resulting in a thick glial scar and consequently a poor tissue-electrode coupling in the chronic phase. We hypothesize, that this adverse effect might be remedied by probes compliant to the soft brain tissue, i.e., replacing rigid electrodes by flexible ones. Unfortunately, this flexibility comes at the price of a low stiffness, which makes targeted low trauma implantation very challenging. In this study, we demonstrate an adaptable and simple method to implant extremely flexible microprobes even to deep areas of rat's brain. Implantation of flexible probes is achieved by rod supported stereotactic insertion fostered by a hydrogel (2% agarose in PBS) cushion on the exposed skull. We were thus able to implant very flexible micro-probes in 70 rats as deep as the rodent's subthalamic nucleus. This work describes in detail the procedures and steps needed for minimal invasive, but reliable implantation of flexible probes. Frontiers Media S.A. 2013-07-24 /pmc/articles/PMC3721086/ /pubmed/23898266 http://dx.doi.org/10.3389/fneng.2013.00006 Text en Copyright © 2013 Richter, Xie, Schumacher, Loeffler, Kirch, Al-Hasani, Rapoport, Kruse, Moser, Tronnier, Danner and Hofmann. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. |
spellingShingle | Neuroscience Richter, Anja Xie, Yijing Schumacher, Anett Löffler, Susanne Kirch, Robert D. Al-Hasani, Jaafar Rapoport, Daniel H. Kruse, Charli Moser, Andreas Tronnier, Volker Danner, Sandra Hofmann, Ulrich G. A simple implantation method for flexible, multisite microelectrodes into rat brains |
title | A simple implantation method for flexible, multisite microelectrodes into rat brains |
title_full | A simple implantation method for flexible, multisite microelectrodes into rat brains |
title_fullStr | A simple implantation method for flexible, multisite microelectrodes into rat brains |
title_full_unstemmed | A simple implantation method for flexible, multisite microelectrodes into rat brains |
title_short | A simple implantation method for flexible, multisite microelectrodes into rat brains |
title_sort | simple implantation method for flexible, multisite microelectrodes into rat brains |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3721086/ https://www.ncbi.nlm.nih.gov/pubmed/23898266 http://dx.doi.org/10.3389/fneng.2013.00006 |
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